Quantitative proteomic analysis reveals that the Rap1/MAPK/ERK pathway is inhibited through selenomethionine strengthening antioxidant activity
Quantitative proteomic analysis reveals that the Rap1/MAPK/ERK pathway is inhibited through selenomethionine strengthening antioxidant activity
复制标题
定量蛋白质组学分析表明，Rap1/MAPK/ERK 通路通过硒代蛋氨酸增强抗氧化活性而受到抑制
DOI：
10.1007/s10534-019-00229-w
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发表时间：
2019-12
期刊：
影响因子：
3.5
通讯作者：
Rui Wu
中科院分区：
文献类型：
--
作者：
Zhe Liu;Feng Zhang;Lina Cui;Jihong Wang;Ping Lu;Rui Zhao;Hua Zhang;Jianfa Wang;Chunqiu Li;Rui Wu
为探讨硒蛋氨酸(SeMet)对雏鸡骨骼肌蛋白质组的影响，对36只雏鸡饲喂SeMet 35d。将72只1日龄肉用仔鸡随机分为两组(n = 36只/组)：对照组(C组)和添加SeMet组(SeMet组)。基础日粮(阴性对照组)和赛米特饲喂组雏鸡骨骼肌中的硒含量分别为0.010 mg/kg和0.40 mg/kg。采用等压相对和绝对定量标记(ITRAQ)结合LC-MS/MS分析方法对两组患者的骨骼肌进行分析。这项蛋白质组学分析确定了两组之间差异表达的蛋白质。分析了35天时来自SEMET组和对照组(C)的3564个蛋白质。ITRAQ分析发现，在SeMet组中有86个蛋白质有差异表达，其中38个表达上调，48个表达下调。这些差异蛋白后来被鉴定为主要参与抗氧化剂和酶调节活性。荧光定量聚合酶链式反应(QPCR)和Western印迹分析与iTRAQ鉴定结果一致。我们的工作中发现的差异表达蛋白(DEP)可能是与雏鸡摄入SeMet相关的特异性生物标志物。SeMet的摄入可能通过Rap1/丝裂原活化蛋白激酶(MAPK)/细胞外信号调节激酶(ERK)信号通路增强抗氧化活性。
To investigate the influence on the proteome of chicken skeletal muscles of Selenomethionine (SeMet) use, 36 chicks were fed with SeMet feeding for 35 days. A total of 72 1-day old broiler chicks were randomly allocated into two groups (n = 36/group): the control group (C group), the SeMet supplemented group (SeMet group). The Selenium (Se) concentrations of skeletal muscles from the chicks with basal diet (negative control group) and SeMet feeding were found to be 0.01 mg/kg and 0.40 mg/kg, respectively. The skeletal muscles from the two groups were investigated using isobaric Tags for Relative and Absolute Quantitation (iTRAQ), coupled with liquid chromatography-tandem mass spectrometry (LC–MS/MS) analysis. This proteomic analysis identified proteins that were differentially expressed between the two groups. A total of 3564 proteins from the SeMet and the control (C) groups at 35 days were analyzed. 86 proteins were found by iTRAQ to be differentially expressed in the SeMet group, including 38 up-regulated proteins and 48 down-regulated proteins. These differential proteins were later identified as being mainly involved in antioxidant and enzyme-regulating activities. Fluorescent quantitative PCR(qPCR) and Western blot analyse proved to be consistent with the results of iTRAQ identification. The differentially expressed proteins (DEPs) identified in our work could be specific biomarkers related to SeMet intake in chicks. SeMet intake may strengthen antioxidant activity through Rap1/mitogen-activated protein kinases (MAPK)/extracellular signal-regulated kinase (ERK) signal pathways.
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