Genetic and molecular characterization of three novel S-haplotypes in sour cherry (Prunus cerasus L.).
Genetic and molecular characterization of three novel S-haplotypes in sour cherry (Prunus cerasus L.).
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DOI：
10.1093/jxb/ern172
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发表时间：
2008
影响因子：
6.9
通讯作者：
Iezzoni AF
中科院分区：
文献类型：
--
作者：
Tsukamoto T;Potter D;Tao R;Vieira CP;Vieira J;Iezzoni AF
四倍体酸樱桃表现出配子体自交不亲和性（GSI），其中自交花粉排斥的特异性分别由花柱和花粉特异性基因S-RNase和SFB（S单倍型特异性F-box蛋白基因）的等位基因控制。由于酸樱桃选择可以是自交亲和（SC）或自交不亲和（SI），多倍体本身不会导致SC。相反，酸樱桃中SI的基因型依赖性损失是由于非功能性S-单倍型的积累。酸樱桃2x花粉中两个或更多个非功能性S-单倍型的存在使得花粉SC。来自酸樱桃的两个新的S-单倍型S33和S34，其被推测是由P. fruticosa物种亲本、第三S-单倍型S35的完整S-RNase和SFB序列以及两个先前鉴定的甜樱桃S-单倍型的存在贡献的，这里描述S14和S16。遗传分离数据表明，S16-，S33-，S34-和S35-单倍型存在于酸樱桃是完全功能的。这一结果与我们以前的发现一致，即“异等位基因”花粉在酸樱桃中不相容。系统发育分析的SFB和S-RNase序列从现有的李属物种揭示，S-单倍型之间的关系显示没有对应的已知的生物体的关系在任何分类学水平内李属，表明在S-位点的多态性一直保持在整个属的进化。此外，SFB序列之间的系统发育关系通常是不一致的S-RNase序列之间相同的S-单倍型。与这些结果相容的假设进行了讨论。
Tetraploid sour cherry (Prunus cerasus L.) exhibits gametophytic self-incompatibility (GSI) whereby the specificity of self-pollen rejection is controlled by alleles of the stylar and pollen specificity genes, S-RNase and SFB (S haplotype-specific F-box protein gene), respectively. As sour cherry selections can be either self-compatible (SC) or self-incompatible (SI), polyploidy per se does not result in SC. Instead the genotype-dependent loss of SI in sour cherry is due to the accumulation of non-functional S-haplotypes. The presence of two or more non-functional S-haplotypes within sour cherry 2x pollen renders that pollen SC. Two new S-haplotypes from sour cherry, S33 and S34, that are presumed to be contributed by the P. fruticosa species parent, the complete S-RNase and SFB sequences of a third S-haplotype, S35, plus the presence of two previously identified sweet cherry S-haplotypes, S14 and S16 are described here. Genetic segregation data demonstrated that the S16-, S33-, S34-, and S35-haplotypes present in sour cherry are fully functional. This result is consistent with our previous finding that ‘hetero-allelic’ pollen is incompatible in sour cherry. Phylogenetic analyses of the SFB and S-RNase sequences from available Prunus species reveal that the relationships among S-haplotypes show no correspondence to known organismal relationships at any taxonomic level within Prunus, indicating that polymorphisms at the S-locus have been maintained throughout the evolution of the genus. Furthermore, the phylogenetic relationships among SFB sequences are generally incongruent with those among S-RNase sequences for the same S-haplotypes. Hypotheses compatible with these results are discussed.
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影响因子：
64.8
作者：
ANDERSON, MA;CORNISH, EC;CLARKE, AE
通讯作者：
CLARKE, AE
影响因子：
56.9
作者：
Richman, AD;Uyenoyama, MK;Kohn, JR
通讯作者：
Kohn, JR
影响因子：
5.4
作者：
Boskovic, RI;Wolfram, B;Sonneveld, T
通讯作者：
Sonneveld, T
影响因子：
1.9
作者：
Potter, D.;Eriksson, T.;Campbell, C. S.
通讯作者：
Campbell, C. S.
影响因子：
64.8
作者：
Sijacic, P;Wang, X;Kao, TH
通讯作者：
Kao, TH