Spatial organization of NG2 glial cells and astrocytes in rat hippocampal CA1 region.
Spatial organization of NG2 glial cells and astrocytes in rat hippocampal CA1 region.
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大鼠海马 CA1 区 NG2 胶质细胞和星形胶质细胞的空间组织
DOI：
10.1002/hipo.22232
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发表时间：
2014-04
期刊：
影响因子：
3.5
通讯作者：
Zhou, Min
中科院分区：
文献类型：
--
作者：
Xu, Guangjin;Wang, Wei;Zhou, Min
与星形胶质细胞相似，NG 2神经胶质细胞均匀分布在中枢神经系统（CNS）中。然而，很少有人知道的空间关系，也不是这两个星形胶质细胞亚型之间的功能相互作用。共聚焦形态学分析显示，NG 2免疫染色细胞在大鼠海马CA 1区空间上组织为域，每个NG 2胶质域占据约178，364 μm3的空间体积。NG 2神经胶质细胞和星形胶质细胞的突起广泛重叠;每个NG 2神经胶质结构域与来自5.8 ± 0.4个相邻星形胶质细胞的突起交错，而每个星形胶质细胞结构域容纳来自4.5 ± 0.3个邻接NG 2神经胶质细胞的突起。在CA 1层辐射，形态学鉴定的胶质细胞的细胞体往往出现直接的体细胞-体细胞接触，称为双峰。我们使用双重斑片记录和记录后NG 2/GFAP双重染色来确定这些双联体的胶质细胞身份。我们发现，在44对中，50%是NG 2胶质细胞-星形胶质细胞对，而另外38.6%和11.4%分别是星形胶质细胞-星形胶质细胞和NG 2胶质细胞-NG 2胶质细胞对。在双膜片记录中，星形胶质细胞-NG 2胶质细胞或NG 2胶质细胞-NG 2胶质细胞双联体中既未检测到电耦合，也未检测到细胞间生物细胞素转移。总之，虽然NG 2胶质细胞和星形胶质细胞不是间隙连接偶联的，但它们的细胞体和突起广泛交织。这项研究揭示的解剖和生理关系，将有助于未来的研究，以了解NG 2胶质细胞和星形胶质细胞之间的代谢耦合和功能的沟通。
Similar to astrocytes, NG2 glial cells are uniformly distributed in the central nervous system (CNS). However, little is known about the interspatial relationship, nor the functional interactions between these two star-shaped glial subtypes. Confocal morphometric analysis showed that NG2 immunostained cells are spatially organized as domains in rat hippocampal CA1 region and that each NG2 glial domain occupies a spatial volume of ~ 178, 364 μm3. The processes of NG2 glia and astrocytes overlap extensively; each NG2 glial domain interlaces with the processes deriving from 5.8 ± 0.4 neighboring astrocytes, while each astrocytic domain accommodates processes stemming from 4.5 ± 0.3 abutting NG2 glia. In CA1 stratum radiatum, the cell bodies of morphologically identified glial cells often appear to make direct somatic-somata contact, termed as doublets. We used dual patch recording and post-recording NG2/GFAP double staining to determine the glial identities of these doublets. We show that among 44 doublets, 50% were NG2 glia-astrocyte pairs, while another 38.6% and 11.4% were astrocyte-astrocyte and NG2 glia-NG2 glia pairs, respectively. In dual patch recording, neither electrical coupling nor intercellular biocytin transfer was detected in astrocyte-NG2 glia or NG2 glia-NG2 glia doublets. Altogether, although NG2 glia and astrocytes are not gap junction coupled, their cell bodies and processes are interwoven extensively. The anatomical and physiological relationships revealed in this study should facilitate future studies to understand the metabolic coupling and functional communication between NG2 glia and astrocytes.
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影响因子：
16.2
作者：
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通讯作者：
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影响因子：
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