Integrated omics data of two annual ryegrass (Lolium multiflorum L.) genotypes reveals core metabolic processes under drought stress.
Integrated omics data of two annual ryegrass (Lolium multiflorum L.) genotypes reveals core metabolic processes under drought stress.
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DOI：
10.1186/s12870-018-1239-z
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发表时间：
2018-01-30
影响因子：
5.3
通讯作者：
Zhang X
中科院分区：
文献类型：
--
作者：
Pan L;Meng C;Wang J;Ma X;Fan X;Yang Z;Zhou M;Zhang X
一年生黑麦草（Lolium multiflorum L.）是一种具有重要商业价值、分布广泛的饲料作物，在全世界范围内用于生产干草和青贮饲料。干旱一直是其生产的严重环境限制。然而，只有少数研究探讨了短期干旱胁迫对一年生黑麦草的影响。本研究的目的是探索胁迫诱导的核心代谢过程如何增强一年生黑麦草的耐旱性或对干旱的适应。我们分析了两种一年生黑麦草基因型的转录组、蛋白质组和代谢组：抗旱基因型“Abundant 10”和干旱敏感基因型“Adrenalin 11”。我们鉴定了差异表达的代谢物及其相应的蛋白质和转录物，它们参与 23 个核心代谢过程，以应对短期干旱胁迫。建立蛋白质-基因-代谢物相关网络来揭示抗旱一年生黑麦草转录本、蛋白质和代谢物表达之间的关系。此外，利用整合代谢途径观察与氨基酸、脂质、碳水化合物缀合物、核苷、生物碱及其衍生物、吡啶及其衍生物水平相对应的酶的变化。由此产生的组学数据强调了 23 个核心代谢过程对于增强一年生黑麦草的耐旱性或适应干旱的重要性。使用 MCoA 和相关性分析推断调控网络，以揭示转录本、蛋白质和代谢物表达之间的关系，突出这些核心代谢途径的相应元素。我们的结果为抗旱的分子机制提供了有价值的见解，并代表了提高一年生黑麦草抗旱性的有前途的策略。本文的在线版本 (10.1186/s12870-018-1239-z) 包含补充材料，可供授权用户使用。
Annual ryegrass (Lolium multiflorum L.) is a commercially important, widely distributed forage crop that is used in the production of hay and silage worldwide. Drought has been a severe environmental constraint in its production. Nevertheless, only a handful of studies have examined the impact of short-term drought stress on annual ryegrass. The aim of this study was to explore how stress-induced core metabolic processes enhance drought tolerance, or adaptation to drought, in annual ryegrass. We profiled the transcriptomes, proteomes, and metabolomes of two annual ryegrass genotypes: the drought-resistant genotype “Abundant 10” and drought-susceptible genotype “Adrenalin 11.” We identified differentially expressed metabolites and their corresponding proteins and transcripts that are involved in 23 core metabolic processes, in response to short-term drought stress. Protein–gene–metabolite correlation networks were built to reveal the relationships between the expression of transcripts, proteins, and metabolites in drought-resistant annual ryegrass. Furthermore, integrated metabolic pathways were used to observe changes in enzymes corresponding with levels of amino acids, lipids, carbohydrate conjugates, nucleosides, alkaloids and their derivatives, and pyridines and their derivatives. The resulting omics data underscored the significance of 23 core metabolic processes on the enhancement of drought tolerance or adaptation to drought in annual ryegrass. The regulatory networks were inferred using MCoA and correlation analysis to reveal the relationships among the expression of transcripts, proteins, and metabolites that highlight the corresponding elements of these core metabolic pathways. Our results provide valuable insight into the molecular mechanisms of drought resistance, and represent a promising strategy toward the improvement of drought tolerance in annual ryegrass. The online version of this article (10.1186/s12870-018-1239-z) contains supplementary material, which is available to authorized users.
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影响因子：
1.9
作者：
Jablonska-Trypuc, Agata;Pankiewicz, Walentyn;Czerpak, Romuald
通讯作者：
Czerpak, Romuald
影响因子：
4.3
作者：
Bettaieb, I.;Zakhama, N.;Marzouk, B.
通讯作者：
Marzouk, B.
影响因子：
7.2
作者：
Hanson, Johannes;Hanssen, Micha;Smeekens, Sjef
通讯作者：
Smeekens, Sjef
影响因子：
2.4
作者：
Borawska-Jarmulowicz, B.;Mastalerczuk, G.;Kalaji, M. H.
通讯作者：
Kalaji, M. H.
影响因子：
7.4
作者：
Liu, JP;Zhu, JK
通讯作者：
Zhu, JK