The transcriptome of lae1 mutants of Trichoderma reesei cultivated at constant growth rates reveals new targets of LAE1 function.
The transcriptome of lae1 mutants of Trichoderma reesei cultivated at constant growth rates reveals new targets of LAE1 function.
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以恒定生长速率培养的里氏木霉 lae1 突变体的转录组揭示了 LAE1 功能的新靶点。
DOI：
10.1186/1471-2164-15-447
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发表时间：
2014-06-09
期刊：
影响因子：
4.4
通讯作者：
Kubicek CP
中科院分区：
文献类型：
--
作者：
Fekete E;Karaffa L;Karimi Aghcheh R;Németh Z;Fekete E;Orosz A;Paholcsek M;Stágel A;Kubicek CP
推测的甲基转移酶LaeA是影响几种真菌中多个次级代谢物基因簇表达的全局调节剂。在里氏木霉（Trichoderma reesei）中，其同源基因LAE1似乎主要调控与提高环境竞争适应性相关的基因，包括纤维素酶和多糖水解酶的表达。然而，到目前为止，所有与LaeA/LAE1功能相关的研究都有一个缺点，即各自的功能丧失和过表达突变体表现出不同的生长速度。因此，LaeA/LAE1的一些特性可能仅仅是由于生长速率的变化。我们将T. reesei，一个Δlae1突变体和一个lae1过表达菌株在葡萄糖的趋化因子中培养成两种不同的生长速率（0.075和0.020 h-1），这两种生长速率分别类似于抑制和去抑制条件下的生长速率。在这些条件下，调节LAE1表达的作用主要体现在Δlae1突变体中，而过表达菌株与亲本菌株差异不大。LAE1对先前确定的一些基因类别（多酮合成酶、异核不相容蛋白、pth11受体）的表达的影响已得到证实，但除此之外，gcn5 - n -乙酰转移酶、氨基酸渗透酶和黄素单加氧酶被确定为迄今为止未知的LAE1作用的主要靶点。LAE1还通过生长速率干扰了几个基因的表达调控。在两种生长条件下，在Δlae1突变体中差异表达的基因中，约有十分之一被发现聚集在基因组中，但没有特定的基因组与这种现象相关。我们的数据表明，以T. reesei LAE1为模型，在恒定生长速率下对调控突变体的转录组研究可以对各自调控体的生理作用产生新的见解。本文的在线版本（doi:10.1186/1471-2164-15-447）包含补充材料，可供授权用户使用。
The putative methyltransferase LaeA is a global regulator that affects the expression of multiple secondary metabolite gene clusters in several fungi. In Trichoderma reesei, its ortholog LAE1 appears to predominantly regulate genes involved in increasing competitive fitness in its environment, including expression of cellulases and polysaccharide hydrolases. A drawback in all studies related to LaeA/LAE1 function so far, however, is that the respective loss-of-function and overexpressing mutants display different growth rates. Thus some of the properties attributed to LaeA/LAE1 could be simply due to changes of the growth rate. We cultivated T. reesei, a Δlae1 mutant and a lae1-overexpressing strain in chemostats on glucose at two different growth rates (0.075 and 0.020 h-1) which resemble growth rates at repressing and derepressing conditions, respectively. Under these conditions, the effect of modulating LAE1 expression was mainly visible in the Δlae1 mutant, whereas the overexpressing strain showed little differences to the parent strain. The effect on the expression of some gene categories identified earlier (polyketide synthases, heterokaryon incompatibility proteins, PTH11-receptors) was confirmed, but in addition GCN5-N-acetyltransferases, amino acid permeases and flavin monooxygenases were identified as so far unknown major targets of LAE1 action. LAE1 was also shown to interfere with the regulation of expression of several genes by the growth rate. About a tenth of the genes differentially expressed in the Δlae1 mutant under either growth condition were found to be clustered in the genome, but no specific gene group was associated with this phenomenon. Our data show that – using T. reesei LAE1 as a model - the investigation of transcriptome in regulatory mutants at constant growth rates leads to new insights into the physiological roles of the respective regulator. The online version of this article (doi:10.1186/1471-2164-15-447) contains supplementary material, which is available to authorized users.
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影响因子：
4.4
作者：
Arvas M;Pakula T;Smit B;Rautio J;Koivistoinen H;Jouhten P;Lindfors E;Wiebe M;Penttilä M;Saloheimo M
通讯作者：
Saloheimo M
DOI：
10.1534/g3.112.005389
发表时间：
2013-03
期刊：
G3 (Bethesda, Md.)
影响因子：
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通讯作者：
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作者：
Ivanova C;Bååth JA;Seiboth B;Kubicek CP
通讯作者：
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影响因子：
2.8
作者：
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影响因子：
2.5
作者：
Chang, Perng-Kuang;Scharfenstein, Leslie L.;Ingber, Bruce F.
通讯作者：
Ingber, Bruce F.