Human serum albumin-coated lipid nanoparticles for delivery of siRNA to breast cancer.
Human serum albumin-coated lipid nanoparticles for delivery of siRNA to breast cancer.
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DOI：
10.1016/j.nano.2012.03.008
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发表时间：
2013-01
影响因子：
5.4
通讯作者：
Lee, Robert J.
中科院分区：
文献类型：
--
作者：
Piao, Longzhu;Li, Hong;Teng, Lesheng;Yung, Bryant C.;Sugimoto, Yasuro;Brueggemeier, Robert W.;Lee, Robert J.
Human serum albumin (HSA)-coated lipid nanoparticles (HSA-LNPs) loaded with phrGFP-targeted siRNA (HSA-LNPs-siRNA) were prepared and evaluated for gene downregulation effect in phrGFP-transfected breast cancer cells and the corresponding xenograft tumor model. HSA-LNPs-siRNA were successfully prepared with a particle size of 79.5±5.5 nm. In phrGFP-transfected MCF-7 cells, HSA-LNPs-siRNA significantly decreased cell fluorescence even in the presence of fetal bovine serum (FBS). Moreover, cell fluorescence and phrGFP mRNA expression were significantly downregulated by HSA-LNPs-siRNA in phrGFP-transfected MCF-7, MDA-MB-231, and SK-BR-3 cells in comparison with control or HSA-LNPs-siRNA (scrambled). In phrGFP-transfected MCF-7 xenograft tumor model, tumor fluorescence was significantly decreased after three IV administrations of HSA-LNPs-siRNA at a dose of 3 mg/kg in comparison with siRNA alone. HSA-LNPs-siRNA demonstrated a superior pharmacokinetic profile in comparison with siRNA at a dose of 1mg/kg. These results show that the novel nonviral carrier, HSA-LNPs, may be used for the delivery of siRNA to breast cancer cells.
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