Genome-wide expression profiling of soybean two-component system genes in soybean root and shoot tissues under dehydration stress.
Genome-wide expression profiling of soybean two-component system genes in soybean root and shoot tissues under dehydration stress.
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DOI：
10.1093/dnares/dsq032
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发表时间：
2011-02
期刊：
影响因子：
--
通讯作者：
Tran LS
中科院分区：
文献类型：
--
作者：
Le DT;Nishiyama R;Watanabe Y;Mochida K;Yamaguchi-Shinozaki K;Shinozaki K;Tran LS
双组分系统（TCSs）在植物适应环境胁迫中起着至关重要的作用。为了鉴定参与干旱胁迫响应调控的大豆TCS基因，我们对遭受脱水的植物中所有83个假定的TCS基因进行了组织特异性表达谱分析。在水分充足的条件下，大多数大豆TCS基因在根组织中表达量更高。此外，在根和茎中都观察到TCS基因转录本丰度的高度变异性。在脱水条件下，TCS基因在茎中的响应比在根中更强烈。进一步分析表明，受脱水抑制的TCS基因比受诱导的多50%。具体而言，有18个基因被诱导2倍或更多，而33个基因被脱水下调至少2倍。假定参与细胞分裂素和乙烯信号传导的TCS基因对脱水有强烈反应，这表明不同激素和胁迫途径之间存在相互作用。我们的研究首次初步了解了大豆TCSs在响应脱水时其功能背后的复杂调控作用。此外，这些系统的表达分析确定了优秀的脱水响应候选基因，以进一步阐明大豆TCS在干旱响应中的功能，并使转基因大豆的耐旱性得到提高。
Two-component systems (TCSs) play vital functions in the adaptation of plants to environmental stresses. To identify soybean TCS genes involved in the regulation of drought stress response, we performed tissue-specific expression profiling of all 83 putative TCS genes in plants subjected to dehydration. Under well-watered conditions, the majority of soybean TCS genes were expressed higher in the root tissues. Additionally, a high variability in transcript abundance was observed for the TCS genes in both roots and shoots. Under dehydration, TCS genes were more responsive in shoots than in roots. Further analysis indicated that 50% more TCS genes were repressed by dehydration than induced. Specifically, 18 genes were induced by 2-fold or more, whereas 33 genes were down-regulated at least 2-fold by dehydration. TCS genes putatively involved in cytokinin and ethylene signallings strongly responded to dehydration, suggesting that crosstalk exists between different hormonal and stress pathways. Our study provides the first glance into the complex regulatory roles of soybean TCSs underlying their functions in response to dehydration. Additionally, these systematic expression analyses identified excellent dehydration-responsive candidate genes to further clarify soybean TCS functions in drought response and to enable the development of improved drought tolerance in transgenic soybeans.
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影响因子：
7.3
作者：
Huang, Jin-Guang;Yang, Mei;Zheng, Cheng-Chao
通讯作者：
Zheng, Cheng-Chao
影响因子：
2.9
作者：
Karan, Ratna;Singla-Pareek, Sneh L.;Pareek, Ashwani
通讯作者：
Pareek, Ashwani
影响因子：
7.2
作者：
Libault, Marc;Farmer, Andrew;Stacey, Gary
通讯作者：
Stacey, Gary
影响因子：
7.4
作者：
Pareek, Ashwani;Singh, Anupama;Singla-Pareek, Sneh L.
通讯作者：
Singla-Pareek, Sneh L.
DOI：
10.1093/dnares/dsp023
发表时间：
2009-12
期刊：
DNA research : an international journal for rapid publication of reports on genes and genomes
影响因子：
--
作者：
Mochida K;Yoshida T;Sakurai T;Yamaguchi-Shinozaki K;Shinozaki K;Tran LS
通讯作者：
Tran LS