Marine Microbial Gene Abundance and Community Composition in Response to Ocean Acidification and Elevated Temperature in Two Contrasting Coastal Marine Sediments.
Marine Microbial Gene Abundance and Community Composition in Response to Ocean Acidification and Elevated Temperature in Two Contrasting Coastal Marine Sediments.
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海洋微生物基因的丰度和社区组成，以响应海洋酸化和温度升高，这两个对比沿海海洋沉积物。
DOI：
10.3389/fmicb.2017.01599
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发表时间：
2017
影响因子：
5.2
通讯作者：
Stahl H
中科院分区：
文献类型：
--
作者：
Currie AR;Tait K;Parry H;de Francisco-Mora B;Hicks N;Osborn AM;Widdicombe S;Stahl H
海洋生态系统面临一系列人类引起的气候压力，特别是气候变化导致的碳酸盐化学变化和海面温度升高。需要更多的研究工作来减少全球范围变暖和酸化对底栖微生物群落影响的不确定性，底栖微生物群落驱动沉积生物地球化学循环。在这项研究中，利用泥质和沙质沿海沉积物进行了中生态实验，以研究二氧化碳浓度升高（750 ppm CO2）和温度升高（环境温度+4°C）对分类和功能微生物基因丰度的独立和交互影响。使用特定的定量 PCR 引物来靶向两种沉积物类型中的古菌、细菌和蓝藻/叶绿体 16S rRNA。氮循环基因古菌和细菌氨单加氧酶 (amoA) 和细菌亚硝酸还原酶 (nirS) 专门用于识别微生物基因丰度的变化以及对氮循环的潜在影响。在泥质沉积物中，28天后，微生物基因丰度（包括amoA和nirS基因）在升高的温度下增加，在升高的CO2下减少，同时伴随着群落组成的变化。相比之下，联合应激源处理在整个实验过程中显示出非加性效应，微生物基因丰度较低。沙质沉积物中微生物群落的反应不太明显，最明显的反应是古菌基因丰度随着时间的推移对环境压力的反应。在沙质沉积物的联合应激处理中，16S rRNA 基因（amoA 和 nirS）丰度较低。我们的研究结果表明，海洋底栖微生物，特别是泥质沉积物中的微生物，很容易受到海洋碳酸盐化学和海水温度变化的影响，这最终可能对关键的底栖生物地球化学循环产生影响。
Marine ecosystems are exposed to a range of human-induced climate stressors, in particular changing carbonate chemistry and elevated sea surface temperatures as a consequence of climate change. More research effort is needed to reduce uncertainties about the effects of global-scale warming and acidification for benthic microbial communities, which drive sedimentary biogeochemical cycles. In this research, mesocosm experiments were set up using muddy and sandy coastal sediments to investigate the independent and interactive effects of elevated carbon dioxide concentrations (750 ppm CO2) and elevated temperature (ambient +4°C) on the abundance of taxonomic and functional microbial genes. Specific quantitative PCR primers were used to target archaeal, bacterial, and cyanobacterial/chloroplast 16S rRNA in both sediment types. Nitrogen cycling genes archaeal and bacterial ammonia monooxygenase (amoA) and bacterial nitrite reductase (nirS) were specifically targeted to identify changes in microbial gene abundance and potential impacts on nitrogen cycling. In muddy sediment, microbial gene abundance, including amoA and nirS genes, increased under elevated temperature and reduced under elevated CO2 after 28 days, accompanied by shifts in community composition. In contrast, the combined stressor treatment showed a non-additive effect with lower microbial gene abundance throughout the experiment. The response of microbial communities in the sandy sediment was less pronounced, with the most noticeable response seen in the archaeal gene abundances in response to environmental stressors over time. 16S rRNA genes (amoA and nirS) were lower in abundance in the combined stressor treatments in sandy sediments. Our results indicated that marine benthic microorganisms, especially in muddy sediments, are susceptible to changes in ocean carbonate chemistry and seawater temperature, which ultimately may have an impact upon key benthic biogeochemical cycles.
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影响因子：
5.1
作者：
Bühring, SI;Elvert, M;Witte, U
通讯作者：
Witte, U
影响因子：
2.4
作者：
Black, KS;Tolhurst, TJ;Hagerthey, SE
通讯作者：
Hagerthey, SE
影响因子：
1.4
作者：
Degerman, Rickard;Dinasquet, Julie;Andersson, Agneta
通讯作者：
Andersson, Agneta
影响因子：
3
作者：
Dickson, AG;Afghan, JD;Anderson, GC
通讯作者：
Anderson, GC
影响因子：
11
作者：
Arandia-Gorostidi, Nestor;Weber, Peter K.;Mayali, Xavier
通讯作者：
Mayali, Xavier