Topography and time course of changes in spinal neuropeptide Y immunoreactivity after spared nerve injury.
Topography and time course of changes in spinal neuropeptide Y immunoreactivity after spared nerve injury.
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DOI：
10.1016/j.neuroscience.2009.10.052
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发表时间：
2010-02-03
期刊：
影响因子：
3.3
通讯作者：
Taylor, B. K.
中科院分区：
文献类型：
--
作者：
Intondi, A. B.;Zadina, J. E.;Zhang, X.;Taylor, B. K.
我们使用一种新的计算机辅助方法来精确定位并有效量化在胫神经和腓总神经（从而保留腓总神经损伤，SNI）、胫神经或腓总神经和腓总神经横断后沿 L4 背角中外侧轴的 NPY 免疫反应性（NPY-ir）的增加。 SNI 两周后，胫骨和腓骨神经支配区域的 NPY-ir 增加；然而，中央外侧区域（受幸存的腓肠神经支配）的 NPY-ir 与 SHAM 的区域没有区别。相反，横断腓肠神经和腓总神经会导致中央外侧区域的 NPY-ir 增加，而内侧区域（由胫神经支配）不受影响。所有神经损伤都会增加背根神经节 (DRG) 和股薄核 (NG) 中的 NPY-ir。到第 24 周时，背角 NPY-ir 的上调以及对寒冷和有害机械刺激的过度反应都得到了解决。相反，DRG 和 NG 中的 NPY-ir 以及对无害静态机械刺激的超敏反应在 24 周内没有消退。在这段时间内，NPY 免疫反应性 DRG 神经元的平均横截面积增加了 150 μm2。我们得出的结论是，SNI 后 NPY 的上调仅限于背角的内侧区域，因此不能直接作用于更外侧（腓肠）区域内的突触来控制腓肠神经过敏。相反，我们认为内侧背角的 NPY 通过中断脊髓和脊髓上水平的中枢敏化和感觉信号整合机制来强直地抑制超敏反应。
We used a new computer-assisted method to precisely localize and efficiently quantify increases in NPY immunoreactivity (NPY-ir) along the mediolateral axis of the L4 dorsal horn following transection of either the tibial and common peroneal nerves (thus sparing the sural branch, spared nerve injury, SNI), the tibial nerve, or the common peroneal and sural nerves. Two weeks after SNI, NPY-ir increased within the tibial and peroneal innervation territories; however, NPY-ir in the central-lateral region (innervated by the spared sural nerve) was indistinguishable from that of SHAM. Conversely, transection of the sural and common peroneal nerved induced an increase in NPY-ir in the central-lateral region, while leaving the medial region (innervated by the tibial nerve) unaffected. All nerve injuries increased NPY-ir in dorsal root ganglia (DRG) and nucleus gracilis (NG). By 24 wk, both NPY-ir up-regulation in the dorsal horn and hyper-responsivity to cold and noxious mechanical stimuli had resolved. Conversely, NPY-ir in DRG and NG, and hypersensitivity to non-noxious static mechanical stimuli, did not resolve within 24 wk. Over this time course, the average cross-sectional area of NPY-immunoreactive DRG neurons increased by 150 μm2. We conclude that the up-regulation of NPY after SNI is restricted to medial zones of the dorsal horn, and therefore cannot act directly upon synapses within the more lateral (sural) zones to control sural nerve hypersensitivity. Instead, we suggest that NPY in the medial dorsal horn tonically inhibits hypersensitivity by interrupting mechanisms of central sensitization and integration of sensory signals at the spinal and supraspinal levels.
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影响因子：
2.5
作者：
SWETT, JE;WOOLF, CJ
通讯作者：
WOOLF, CJ
影响因子：
3
作者：
Smith, Peter A.;Moran, Timothy D.;Taylor, Bradley K.
通讯作者：
Taylor, Bradley K.
影响因子：
2.9
作者：
Brumovsky, PR;Bergman, E;Villar, MJ
通讯作者：
Villar, MJ
影响因子：
--
作者：
SCHMALBRUCH, H
通讯作者：
SCHMALBRUCH, H
影响因子：
15.1
作者：
Beggs, Simon;Salter, Michael W.
通讯作者：
Salter, Michael W.