Antibody-capped mesoporous nanoscopic materials: design of a probe for the selective chromo-fluorogenic detection of finasteride.
Antibody-capped mesoporous nanoscopic materials: design of a probe for the selective chromo-fluorogenic detection of finasteride.
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DOI：
10.1002/open.201100008
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发表时间：
2012-12
期刊：
影响因子：
2.3
通讯作者：
Amoros, Pedro
中科院分区：
文献类型：
--
作者：
Climent, Estela;Martinez-Manez, Ramon;Maquieira, Angel;Sancenon, Felix;Dolores Marcos, M.;Brun, Eva M.;Soto, Juan;Amoros, Pedro
为了获得能够在偶联抗体选择性结合的靶分子（抗原）存在的情况下释放被包裹的货物（染料）的递送系统，已经进行了与抗体（AB）偶联作为看门人的带帽介孔二氧化硅纳米颗粒（MSN）的合成。特别制备了负载罗丹明B的微球，并在外表面用合适的N-（t-丁基）-3-氧-(5α,17β)-4-氮杂androst-1-烯-17-羧酰胺（非那雄胺）衍生物进行功能化（S1）。添加抗非那雄胺的多克隆抗体，由于与锚定的半抗原样非那雄胺衍生物相互作用，诱导孔隙封盖，从而获得msn -半抗原- ab纳米颗粒S1-AB。结果发现，在含有非那雄胺的水溶液中加入被盖物S1-AB，导致抗体位移、孔解盖和包覆染料释放。门控物质的反应具有高度选择性，在其他类固醇中，只有非那雄胺能够诱导显着的解锁过程。与非那雄胺相比，非那雄胺代谢物能够释放17%的染料，而外源类固醇睾酮、美替诺酮和16-β-羟stanozolol仅能诱导含有传感固体S1-AB的水悬浮液释放很少的罗丹明B（低于10%）。荧光法检测非那雄胺的检出限低至20 ppb。为了评估该材料在无标记检测非那雄胺方面的可能应用，分离并储存了被封住的材料，得到最终的传感固体s1 - ab - 1。发现它对非那雄胺表现出与新鲜制备的S1-AB相似的行为；即使在两个月后，也没有发现选择性或敏感性的显著下降。此外，为了研究在生物样品中检测非那雄胺的应用，这种“老化”的材料S1-AB-i以市售的空白尿液为基质进行了测试。在含有70%和90%空白尿液的样品中加入一定量的非那雄胺，并用盖帽S1-AB-i测定浓度。回收率为94% ~ 118%。
The synthesis of capped mesoporous silica nanoparticles (MSN) conjugated with an antibody (AB) as a gatekeeper has been carried out in order to obtain a delivery system able to release an entrapped cargo (dye) in the presence of a target molecule (antigen) to which the conjugated antibody binds selectively. In particular, MSN loaded with rhodamine B and functionalized on the external surface with a suitable derivative of N-(t-butyl)-3-oxo-(5α,17β)-4-aza-androst-1-ene-17-carboxamide (finasteride) have been prepared (S1). The addition of polyclonal antibodies against finasteride induced capping of the pores due to the interaction with the anchored hapten-like finasteride derivative to give a MSN–hapten–AB nanoparticle S1-AB. It was found that the addition of capped material S1-AB to water solutions containing finasteride resulted in displacement of the antibody, pore uncapping and entrapped-dye release. The response of the gated material is highly selective, and only finasteride, among other steroids, was able to induce a significant uncapping process. Compared with finasteride, the finasteride metabolite was able to release 17 % of the dye, whereas the exogen steroids testosterone, metenolone and 16-β-hydroxystanozolol only induced very little release of rhodamine B (lower than 10 %) from aqueous suspensions containing sensing solid S1-AB. A detection limit as low as 20 ppb was found for the fluorimetric detection of finasteride. In order to evaluate a possible application of the material for label-free detection of finasteride, the capped material was isolated and stored to give final sensing solid S1-AB-i. It was found to display a similar behavior towards finasteride as to that shown by freshly prepared S1-AB; even after a period of two months, no significant loss of selectivity or sensitivity was noted. Moreover, to study the application for the detection of finasteride in biological samples, this “aged” material, S1-AB-i, was tested using commercially available blank urine as matrix. Samples containing 70 and 90 % blank urine were spiked with a defined amount of finasteride, and the concentration was determined using capped S1-AB-i. Recovery ranges from 94 % to 118 % were reached.
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影响因子：
16.6
作者：
Climent, Estela;Martinez-Manez, Ramon;Amoros, Pedro
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