Chromosomal Behavior during Meiosis in the Progeny of Triticum timopheevii × Hexaploid Wild Oat.
Chromosomal Behavior during Meiosis in the Progeny of Triticum timopheevii × Hexaploid Wild Oat.
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DOI：
10.1371/journal.pone.0126398
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Zhang S
中科院分区：
文献类型：
--
作者：
An H;Hu M;Li P;Geng G;Zhang Q;Zhang S
本研究通过细胞学分析和序贯C带基因组原位杂交（GISH）研究了提莫菲维小麦×六倍体野燕麦F2和F3后代花粉母细胞（PMC）的减数分裂行为。细胞学分析显示，F2和F3后代的染色体数目为28～41条。F2和F3后代在中期I、后期I、后期II和四分体阶段发现大量单价染色体、滞后染色体、染色体桥和微核。 F2 和 F3 后代中每个细胞的单价平均值分别为 3.50 和 2.73，滞后染色体分别为 3.37 和 1.87。 F2和F3后代的PMC减数分裂指数分别为12.22和20.34，表明相当大的遗传不稳定性。连续C显带-GISH分析表明，在后代的中期I和后期I中检测到了来自六倍体野燕麦的一些染色体和片段，表明该后代具有真正的属间杂种起源。外来染色体 6A、7A、3C 和 2D 在从 F2 传递到 F3 的过程中丢失。此外，部分T. timopheevii染色体以单价或滞后染色体的形式出现，这可能是由于亲本之间基因组差异较大，而野燕麦染色体渗入干扰了小麦同源物的正常配对。
The meiotic behavior of pollen mother cells (PMCs) of the F2 and F3 progeny from Triticum timopheevii × hexaploid wild oat was investigated by cytological analysis and sequential C-banding-genomic in situ hybridization (GISH) in the present study. A cytological analysis showed that the chromosome numbers of the F2 and F3 progeny ranged from 28 to 41. A large number of univalents, lagging chromosomes, chromosome bridges and micronuclei were found at the metaphase I, anaphase I, anaphase II and tetrad stages in the F2 and F3 progeny. The averages of univalents were 3.50 and 2.73 per cell, and those of lagging chromosomes were 3.37 and 1.87 in the F2 and F3 progeny, respectively. The PMC meiotic indices of the F2 and F3 progeny were 12.22 and 20.34, respectively, indicating considerable genetic instability. A sequential C-banding-GISH analysis revealed that some chromosomes and fragments from the hexaploid wild oat were detected at metaphase I and anaphase I in the progeny, showing that the progeny were of true intergeneric hybrid origin. The alien chromosomes 6A, 7A, 3C and 2D were lost during transmission from F2 to F3. In addition, partial T. timopheevii chromosomes appeared in the form of univalents or lagging chromosomes, which might result from large genome differences between the parents, and the wild oat chromosome introgression interfered with the wheat homologues’ normally pairing.
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