Multiple forms of BRMS1 are differentially expressed in the MCF10 isogenic breast cancer progression model.
Multiple forms of BRMS1 are differentially expressed in the MCF10 isogenic breast cancer progression model.
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DOI：
10.1007/s10585-008-9216-9
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发表时间：
2009
影响因子：
4
通讯作者：
Welch, Danny R.
中科院分区：
文献类型：
--
作者：
Hurst, Douglas R.;Xie, Yi;Edmonds, Mick D.;Welch, Danny R.
评估BRMS 1转移抑制因子在乳腺癌进展中的mRNA表达水平的临床研究并不一致。本研究的目的是表征乳腺癌进展模型中内源性BRMS 1 mRNA和蛋白的特征。在代表“正常”乳腺上皮细胞（MCF 10A）、癌前乳腺疾病（MCF 10AT）、粉刺导管原位癌（MCF10DCIS.com）和转移性癌（MCF10CAa.1和MCF10CAd.1α）的遗传相关MCF 10细胞系中，使用识别BRMS 1蛋白中不同表位的两种抗体评价BRMS 1蛋白表达。在所有细胞系中检测到特征性的35 kDa BRMS 1蛋白的核表达。由于BRMS 1在转移性MCF 10变体中表达，因此对BRMS 1外显子进行测序以扫描可能的基因突变。BRMS 1是野生型的，除了外显子7中的同义T/C转换。然而，通过RT-PCR检测到可变剪接变体。两种变体BRMS1.v2和BRMS1.v4仅在MCF 10A和AT细胞系中检测到，而BRMS 1和BRMS1.v3在所有细胞系中检测到。这些结果表明，特征性的35 kDa BRMS 1蛋白的表达不足以防止转移。选择性剪接变异体的差异表达表明，在评估临床样本中BRMS 1 mRNA时应谨慎。
Clinical studies evaluating the mRNA expression level of the BRMS1 metastasis suppressor in the progression of breast cancer have not been consistent. The purpose of this study was to characterize endogenous BRMS1 mRNA and protein in a model of the progression of breast cancer. BRMS1 protein expression was evaluated in the genetically related MCF10 cell lines representing ‘normal’ breast epithelial cells (MCF10A), pre-malignant breast disease (MCF10AT), comedo ductal carcinoma in situ (MCF10DCIS.com), and metastatic carcinoma (MCF10CAa.1 and MCF10CAd.1α) with two antibodies that recognize distinct epitopes in the BRMS1 protein. Nuclear expression of the characteristic ∼35 kDa BRMS1 protein was detected in all cell lines. Because BRMS1 was expressed in the metastatic MCF10 variants, the BRMS1 exons were sequenced to scan for possible genetic mutations. BRMS1 was wild-type with the exception of a synonymous T/C transition in exon 7. However, alternatively spliced variants were detected by RT-PCR. Two variants, BRMS1.v2 and BRMS1.v4 were only detected in the MCF10A and AT cell lines, while BRMS1 and BRMS1.v3 were detected in all lines. These results demonstrate that expression of the characteristic ∼35 kDa BRMS1 protein is not sufficient to prevent metastasis. The differential expression of alternative splice variants suggests caution should be taken when evaluating BRMS1 mRNA in clinical samples.
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影响因子：
37.3
作者：
Samant RS;Clark DW;Fillmore RA;Cicek M;Metge BJ;Chandramouli KH;Chambers AF;Casey G;Welch DR;Shevde LA
通讯作者：
Shevde LA
影响因子：
4.8
作者：
Meehan, WJ;Samant, RS;Welch, DR
通讯作者：
Welch, DR
影响因子：
2.5
作者：
Miller, FR
通讯作者：
Miller, FR
影响因子：
4
作者：
Samant, RS;Seraj, MJ;Welch, DR
通讯作者：
Welch, DR
影响因子：
11.5
作者：
Hicks, David G.;Yoder, Brian J.;Casey, Graham
通讯作者：
Casey, Graham