Neuroanatomical studies of period gene expression in the hawkmoth, Manduca sexta
Neuroanatomical studies of period gene expression in the hawkmoth, Manduca sexta
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天蛾天蛾周期基因表达的神经解剖学研究
DOI：
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
K. Siwicki
中科院分区：
文献类型：
--
作者：
S. Wise;N. T. Davis;E. Tyndale;Jocelyne Noveral;M. Folwell;V. Bedian;Ivette F. Emery;K. Siwicki
在天蛾的神经系统，天蛾，细胞表达周期（每）基因的原位杂交和免疫细胞化学方法绘制。洋地黄毒苷标记的核糖核酸探针从1 kb M. sexta/cDNA。将单克隆抗PER抗体产生为从两种M. sexta和黑腹果蝇的cDNA序列。这些试剂揭示了每个基因产物在M.眼睛、视叶、大脑和大脑后复合体。每mRNA的标记在整个大脑的光感受器和神经胶质细胞中，以及在邻近视叶副髓质的100-200个神经元的簇中是突出的。仅在胶质细胞中检测到每mRNA水平的日节律。在大多数神经元和神经胶质细胞的细胞核以及许多感光细胞核中观察到PER样免疫反应。每个大脑半球外侧部的4个神经分泌细胞均显示抗PER抗体的细胞核和细胞质染色。这些细胞被鉴定为Ia 1神经分泌细胞，表达corazonin免疫反应性。Anti‐corazonin标记了它们在大脑中的投射以及它们在心侧体和咽侧体中的神经血管末梢。先前在蚕蛾Anthereae pernyi中描述的四对表达PER的神经分泌细胞可能与M的这些PER/corazonin表达Ia 1细胞同源。六。其他发现，如广泛的核定位的M。sexta PER和神经胶质细胞的节律性表达，是D.这表明PER的某些功能在该鳞翅目物种中可能是保守的。J. Comp.神经元447：366-380，2002.© 2002 Wiley利斯公司
In the nervous system of the hawkmoth, Manduca sexta, cells expressing the period (per)gene were mapped by in situ hybridization and immunocytochemical methods. Digoxigenin‐labeled riboprobes were transcribed from a 1‐kb M. sexta per cDNA. Monoclonal anti‐PER antibodies were raised to peptide antigens translated from both M. sexta and Drosophila melanogaster per cDNAs. These reagents revealed a widespread distribution of per gene products in M. sexta eyes, optic lobes, brains, and retrocerebral complexes. Labeling for per mRNA was prominent in photoreceptors and in glial cells throughout the brain, and in a cluster of 100–200 neurons adjacent to the accessory medulla of the optic lobes. Daily rhythms of per mRNA levels were detected only in glial cells. PER‐like immunoreactivity was observed in nuclei of most neurons and glial cells and in many photoreceptor nuclei. Four neurosecretory cells in the pars lateralis of each brain hemisphere exhibited both nuclear and cytoplasmic staining with anti‐PER antibodies. These cells were positively identified as Ia1 neurosecretory cells that express corazonin immunoreactivity. Anti‐corazonin labeled their projections in the brain and their neurohemal endings in the corpora cardiaca and corpora allata. Four pairs of PER‐expressing neurosecretory cells previously described in the silkmoth, Anthereae pernyi, are likely to be homologous to these PER/corazonin‐expressing Ia1 cells of M. sexta. Other findings, such as widespread nuclear localization of M. sexta PER and rhythmic expression in glial cells, are reminiscient of the period gene of D. melanogaster, suggesting that some functions of per may be conserved in this lepidopteran species. J. Comp. Neurol. 447:366–380, 2002. © 2002 Wiley‐Liss, Inc.
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影响因子：
56.9
作者：
Plautz, JD;Kaneko, M;Kay, SA
通讯作者：
Kay, SA
DOI：
10.1073/pnas.94.8.4092
发表时间：
1997-04-15
影响因子：
11.1
作者：
Emery, IF;Noveral, JM;Siwicki, KK
通讯作者：
Siwicki, KK
影响因子：
2.7
作者：
DAI, JD;MIZOGUCHI, A;GILBERT, LI
通讯作者：
GILBERT, LI
含有数量不断增加的果蝇时钟基因周期和 lacZ 报告基因的转基因的时间和空间表达模式：绘制参与昼夜节律循环的 PER 蛋白元素。
DOI：
10.1523/jneurosci.17-02-00676.1997
发表时间：
1997
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Stanewsky,R;Frisch,B;Brandes,C;Hamblen-Coyle,MJ;Rosbash,M;Hall,JC
通讯作者：
Hall,JC
影响因子：
56.9
作者：
Darlington, TK;Wager-Smith, K;Kay, SA
通讯作者：
Kay, SA