An organophosphonate strategy for functionalizing silicon photonic biosensors.
An organophosphonate strategy for functionalizing silicon photonic biosensors.
复制标题
DOI：
10.1021/la2043153
复制
发表时间：
2012-02-14
期刊：
影响因子：
--
通讯作者：
Ratner DM
中科院分区：
文献类型：
--
作者：
Shang J;Cheng F;Dubey M;Kaplan JM;Rawal M;Jiang X;Newburg DS;Sullivan PA;Andrade RB;Ratner DM
硅光子微环谐振器已经在无标记和低成本生物传感应用中发挥了潜力。然而，这种光学传感平台的长期性能需要强大的表面改性和生物功能化。在此，我们展示了一种基于有机膦酸酯表面涂层和乙烯基砜连接体的缀合策略，以生物功能化硅谐振器用于生物分子传感。为了验证该方法，将一系列聚糖（包括碳水化合物和糖缀合物）固定在二乙烯基砜（DVS）/有机膦酸酯改性的微环上，并用于表征碳水化合物-蛋白质和诺如病毒颗粒相互作用。该生物功能平台能够同时正交检测多种特异性碳水化合物-蛋白质相互作用。此外，该平台在高盐、高pH或低pH溶液多次再生后以及在环境条件下储存1个月后能够重现结合。有机膦酸酯固定化策略的这种显著的稳定性和耐久性将促进硅微型谐振器在各种感测条件下的应用，延长它们的寿命，并最小化存储和递送的成本;这些特性对于开发用于即时护理和分布式诊断以及其他生物医学应用的生物传感器是必需的。此外，该平台证明了其表征碳水化合物介导的宿主-病毒相互作用的能力，为发现新的抗病毒药物以预防传染病提供了一种简便的方法。
Silicon photonic microring resonators have established their potential for label-free and low-cost biosensing applications. However, the long-term performance of this optical sensing platform requires robust surface modification and biofunctionalization. Herein, we demonstrate a conjugation strategy based on an organophosphonate surface coating and vinyl sulfone linker to biofunctionalize silicon resonators for biomolecular sensing. To validate this method, a series of glycans, including carbohydrates and glycoconjugates, were immobilized on divinyl sulfone (DVS)/organophosphonate-modified microrings and used to characterize carbohydrate-protein and norovirus particle interactions. This biofunctional platform was able to orthogonally detect multiple specific carbohydrate-protein interactions simultaneously. Additionally, the platform was capable of reproducible binding after multiple regenerations by high-salt, high-pH or low-pH solutions and after 1-month storage in ambient conditions. This remarkable stability and durability of the organophosphonate immobilization strategy will facilitate the application of silicon microring resonators in various sensing conditions, prolong their lifetime, and minimize the cost for storage and delivery; these characteristics are requisite for developing biosensors for point-of-care and distributed diagnostics and other biomedical applications. In addition, the platform demonstrated its ability to characterize carbohydrate-mediated host-virus interactions, providing a facile method for discovering new anti-viral agents to prevent infectious disease.
登录
查看更多内容
用于定量细胞因子检测和 T 细胞分泌分析的硅光子微环谐振器。
影响因子：
7.4
作者：
Luchansky, Matthew S.;Bailey, Ryan C.
通讯作者：
Bailey, Ryan C.
影响因子：
4.7
作者：
Cheng, Fang;Shang, Jing;Ratner, Daniel M.
通讯作者：
Ratner, Daniel M.
影响因子：
6.4
作者：
Jiang, X;Huang, PW;Pickering, LK
通讯作者：
Pickering, LK
影响因子：
5.4
作者：
Huang, PW;Farkas, T;Xi, J
通讯作者：
Xi, J
影响因子：
3.9
作者：
Adden, Nina;Gamble, Lara J.;Menzel, Henning
通讯作者：
Menzel, Henning