The Functions of β-Defensin in Flounder (Paralichthys olivaceus): Antibiosis, Chemotaxis and Modulation of Phagocytosis.
The Functions of β-Defensin in Flounder (Paralichthys olivaceus): Antibiosis, Chemotaxis and Modulation of Phagocytosis.
复制标题
比目鱼 (Paralichthys olivaceus) β-防御素的功能：抗菌、趋化性和吞噬作用的调节
DOI：
10.3390/biology10121247
复制
发表时间：
2021-11-29
期刊：
影响因子：
4.2
通讯作者：
Zhan W
中科院分区：
文献类型：
--
作者：
Hao X;Chi H;Tang X;Xing J;Sheng X;Zhan W
本研究从牙鲆（Paralichthys olivaceus，fBD）中鉴定了一种新的阴离子β-防御素剪接异构体，并研究了其趋化性、趋化性和吞噬调节作用。同时分析了fBD对胞外陷阱（Extracellular Traps，ETP）抗菌活性的贡献。分析发现，鱼类中的阴离子β-防御素具有与阳离子防御素相同的强大抑菌能力，并且在免疫反应中也发挥着重要作用。本研究为了解阴离子防御素的生物学功能提供了新的视角，阴离子防御素可以作为细胞外陷阱中的重要效应物之一，并有助于免疫应答。大多数防御素是阳离子抗菌肽，对细菌、真菌和包膜病毒具有广谱杀伤活性。但应该认识到，生物体中也存在一些非阳离子β-防御素，需要进一步研究。本研究从牙鲆（Paralichthys olivaceus，fBD）中鉴定了一种新的阴离子β-防御素剪接异构体，并对其趋化性、趋化性和吞噬调节作用进行了研究。此外，还分析了fBD对胞外陷阱（ECTS）抗菌活性的贡献。重组fBD（rfBD）能有效抑制革兰氏阳性菌（S.金黄色葡萄球菌（Micrococcus aureus）、藤黄微球菌（Micrococcus luteus）和革兰氏阴性菌（E.大肠杆菌、溶藻弧菌、鳗弧菌）。间接免疫荧光试验表明，fBD共定位在由白细胞释放的细胞外陷阱。当用rfBD抗体封闭S. aureus和E.大肠埃希菌与大肠埃希菌共孵育后，与对照组相比有增加趋势。流式细胞仪检测结果表明，rfBD具有明显的趋化作用，并能增强白细胞的吞噬活性。总之，本研究提供了新的见解，阴离子防御素的生物学功能，它可以作为一个重要的效应器在细胞外陷阱，并作为一个桥梁之间的天然免疫和获得性免疫的硬骨鱼。
The study identified a new spliced isoform of anionic β-defensin from flounder (Paralichthys olivaceus, fBD) and examined its antibiosis, chemotaxis and modulation of phagocytosis. It also analyzed the contributions of fBD to the antimicrobial activity of extracellular traps (ETs). The analyses found that an anionic β-defensin in fish possesses strong bacteriostatic ability in line with that of cationic defensins and also plays an important role in immune response. This study provides new insights into the biological function of anionic defensins, which can serve as one of the important effectors in extracellular traps and contribute to the immune response. Most defensins are cationic antimicrobial peptides with broad-spectrum killing activity against bacteria, fungi and enveloped viruses. However, it should be recognized that there are some non-cationic β-defensins in organisms, which need to be further studied. In this study, a new spliced isoform of anionic β-defensin from flounder (Paralichthys olivaceus, fBD) was identified, and its antibiosis, chemotaxis and modulation of phagocytosis were examined. In addition, the contributions of fBD to the antimicrobial activity of extracellular traps (ETs) were also analyzed. The recombinant fBD (rfBD) could effectively inhibit the growth of Gram-positive bacteria (S. aureus, Micrococcus luteus) and Gram-negative bacteria (E. coli, V. alginolyticus, V. anguillarum). An indirect immunofluorescence assay showed that the fBD was co-localized in the extracellular traps released by the leukocytes. When the ETs were blocked with antibodies against rfBD, the proliferation of S. aureus and E. coli incubated with ETs tended to increase compared with that in the control group. In addition, the results obtained by flow cytometry showed that the rfBD could significantly chemoattract leukocytes and increase phagocytic activity in vitro. In conclusion, this study provides new insights into the biological function of anionic defensins, which can serve as one of the important effectors in extracellular traps and as a bridge between innate and adaptive immunity in teleosts.
登录
查看更多内容
影响因子：
4.7
作者：
Li, Qiang;Zhan, Wenbin;Sheng, Xiuzhen
通讯作者：
Sheng, Xiuzhen
影响因子：
15.9
作者：
GANZ, T;SELSTED, ME;LEHRER, RI
通讯作者：
LEHRER, RI
影响因子：
4.6
作者：
Oliva R;Chino M;Pane K;Pistorio V;De Santis A;Pizzo E;D'Errico G;Pavone V;Lombardi A;Del Vecchio P;Notomista E;Nastri F;Petraccone L
通讯作者：
Petraccone L
影响因子：
4.8
作者：
Lauth, X;Shike, H;Bulet, P
通讯作者：
Bulet, P
影响因子：
4.8
作者：
Cole, AM;Weis, P;Diamond, G
通讯作者：
Diamond, G