Transcriptome analysis of response to Plasmodiophora brassicae infection in the Arabidopsis shoot and root.
Transcriptome analysis of response to Plasmodiophora brassicae infection in the Arabidopsis shoot and root.
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DOI：
10.1186/s12864-017-4426-7
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发表时间：
2018-01-05
期刊：
影响因子：
4.4
通讯作者：
Bonham-Smith PC
中科院分区：
文献类型：
--
作者：
Irani S;Trost B;Waldner M;Nayidu N;Tu J;Kusalik AJ;Todd CD;Wei Y;Bonham-Smith PC
根肿病是由专性寄生物芸苔根肿菌（Plasmodiophora brassicae）引起的一种重要病害。作为一种土传病原菌，芸苔疫霉诱导根中异常组织的产生，导致虫瘿的形成。根感染对宿主植物吸收水分和养分产生负面影响，严重降低其生长和生产力。许多研究强调根肿病对根组织的分子和生理影响。本研究的目的是更好地了解P. brassicae对拟南芥地上部和根组织转录组的影响。在接种A. thaliana是芸苔疫霉的模式寄主。感染和未感染的样品之间的差异表达基因（DEG）的数量是在地上部比在根。在芽和根中，在24 dpi比两个较早的时间点差异调节更多的基因。在茎和根中高度调控的基因主要参与细胞壁化合物、脂质和莽草酸途径代谢物的代谢。在与茉莉酸相关的途径中，几个茉莉酸生物合成基因在地上部和根组织中都上调。编码参与细胞壁修饰、蔗糖和淀粉生物合成的酶以及几类转录因子的基因在茎和根中通常受到不同的调控。这些结果突出了模式宿主拟南芥的地上和地下组织在P. brassicae感染后转录组响应的相似性和差异性。确定了根肿病进展过程中根代谢的主要转录组变化。在拍摄的DEG的概述强调了在地上组织病原体建立和疾病进展后的生理变化。这项研究提供了深入了解主机组织特异性分子反应根肿病的发展，并可能在根肿病标记的发展，更有效的育种策略的应用。本文的在线版本（10.1186/s12864-017-4426-7）包含补充材料，可供授权用户使用。
Clubroot is an important disease caused by the obligate parasite Plasmodiophora brassicae that infects the Brassicaceae. As a soil-borne pathogen, P. brassicae induces the generation of abnormal tissue in the root, resulting in the formation of galls. Root infection negatively affects the uptake of water and nutrients in host plants, severely reducing their growth and productivity. Many studies have emphasized the molecular and physiological effects of the clubroot disease on root tissues. The aim of the present study is to better understand the effect of P. brassicae on the transcriptome of both shoot and root tissues of Arabidopsis thaliana. Transcriptome profiling using RNA-seq was performed on both shoot and root tissues at 17, 20 and 24 days post inoculation (dpi) of A. thaliana, a model plant host for P. brassicae. The number of differentially expressed genes (DEGs) between infected and uninfected samples was larger in shoot than in root. In both shoot and root, more genes were differentially regulated at 24 dpi than the two earlier time points. Genes that were highly regulated in response to infection in both shoot and root primarily were involved in the metabolism of cell wall compounds, lipids, and shikimate pathway metabolites. Among hormone-related pathways, several jasmonic acid biosynthesis genes were upregulated in both shoot and root tissue. Genes encoding enzymes involved in cell wall modification, biosynthesis of sucrose and starch, and several classes of transcription factors were generally differently regulated in shoot and root. These results highlight the similarities and differences in the transcriptomic response of above- and below-ground tissues of the model host Arabidopsis following P. brassicae infection. The main transcriptomic changes in root metabolism during clubroot disease progression were identified. An overview of DEGs in the shoot underlined the physiological changes in above-ground tissues following pathogen establishment and disease progression. This study provides insights into host tissue-specific molecular responses to clubroot development and may have applications in the development of clubroot markers for more effective breeding strategies. The online version of this article (10.1186/s12864-017-4426-7) contains supplementary material, which is available to authorized users.
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影响因子：
2.9
作者：
Jubault, Melanie;Lariagon, Christine;Taconnat, Ludivine;Renou, Jean-Pierre;Gravot, Antoine;Delourme, Regine;Manzanares-Dauleux, Maria J.
通讯作者：
Manzanares-Dauleux, Maria J.
影响因子：
3
作者：
Agarwal, Arati;Kaul, Vijay;Cahill, David M.
通讯作者：
Cahill, David M.
影响因子：
3.5
作者：
Devos, Sylvie;Laukens, Kris;Prinsen, Els
通讯作者：
Prinsen, Els
DOI：
10.1007/s11101-011-9211-7
发表时间：
2011-09
期刊：
Phytochemistry reviews : proceedings of the Phytochemical Society of Europe
影响因子：
--
作者：
Dao TT;Linthorst HJ;Verpoorte R
通讯作者：
Verpoorte R
影响因子：
9.4
作者：
Devos, S;Vissenberg, K;Prinsen, E
通讯作者：
Prinsen, E