Root respiratory burst oxidase homologue-dependent H2O2 production confers salt tolerance on a grafted cucumber by controlling Na+ exclusion and stomatal closure.
Root respiratory burst oxidase homologue-dependent H2O2 production confers salt tolerance on a grafted cucumber by controlling Na+ exclusion and stomatal closure.
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根呼吸爆发氧化酶同系物依赖的 H2O2 产生通过控制钠排除和气孔关闭赋予嫁接黄瓜耐盐性。
DOI：
10.1093/jxb/erx386
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发表时间：
2018-06-19
影响因子：
6.9
通讯作者：
Bie Z
中科院分区：
文献类型：
--
作者：
Niu M;Huang Y;Sun S;Sun J;Cao H;Shabala S;Bie Z
根呼吸爆发氧化酶同系物依赖的 H2O2 产生是通过调节 Na+ 排除和气孔关闭赋予嫁接黄瓜耐盐性的早期信号。通过嫁接到耐盐砧木上可以提高植物的耐盐性。然而，这种现象背后的潜在信号机制仍然很大程度上未知。为了解决这个问题，我们使用了一系列生理和分子技术来研究自嫁接和南瓜嫁接黄瓜植物暴露于 75 mM NaCl 胁迫下的反应。南瓜嫁接显着提高了黄瓜植株的耐盐性，表现为更高的植株干重、叶绿素含量和光化学效率（Fv/Fm）以及更低的叶片Na+含量。盐胁迫导致南瓜嫁接黄瓜的H2O2产量急剧增加，并在盐处理后3小时达到峰值。这种增强伴随着呼吸爆发氧化酶同源物 (RBOH) 基因 RbohD 和 RbohF 的相对表达升高以及更高的 NADPH 氧化酶活性。然而，这种增加在自嫁接植物中延迟了很多，并且两种嫁接组合之间的差异在24小时后消失。南瓜嫁接植物叶片 Na+ 含量的降低是通过根部较高的 Na+ 排除来实现的，这是由质膜 H+-ATP 酶激发的 Na+/H+ 逆向转运蛋白驱动的，较高的质膜 H+-ATP 酶活性和 PMA 和 SOS1 转录水平较高就证明了这一点。此外，在南瓜嫁接的黄瓜植物中还观察到气孔提前关闭，减少了水分流失并维持了植物的水合状态。当南瓜嫁接植物用 NADPH 氧化酶抑制剂二亚苯基碘 (DPI) 预处理时，H2O2 水平显着下降，达到自嫁接植物的水平，导致耐盐性丧失。根部 NADPH 氧化酶介导的 H2O2 信号传导的抑制也消除了南瓜嫁接植物中气孔的快速关闭。我们得出的结论是，南瓜嫁接的黄瓜植物通过涉及根源呼吸爆发氧化酶同系物依赖的 H2O2 产生的机制来提高其耐盐性，从而增强根部对 Na+ 的排除并促进气孔早期关闭。
Root respiratory burst oxidase homologue-dependent H2O2 production serves as an early signal to confer salt tolerance on a grafted cucumber through regulation of Na+ exclusion and stomatal closure. Plant salt tolerance can be improved by grafting onto salt-tolerant rootstocks. However, the underlying signaling mechanisms behind this phenomenon remain largely unknown. To address this issue, we used a range of physiological and molecular techniques to study responses of self-grafted and pumpkin-grafted cucumber plants exposed to 75 mM NaCl stress. Pumpkin grafting significantly increased the salt tolerance of cucumber plants, as revealed by higher plant dry weight, chlorophyll content and photochemical efficiency (Fv/Fm), and lower leaf Na+ content. Salinity stress resulted in a sharp increase in H2O2 production, reaching a peak 3 h after salt treatment in the pumpkin-grafted cucumber. This enhancement was accompanied by elevated relative expression of respiratory burst oxidase homologue (RBOH) genes RbohD and RbohF and a higher NADPH oxidase activity. However, this increase was much delayed in the self-grafted plants, and the difference between the two grafting combinations disappeared after 24 h. The decreased leaf Na+ content of pumpkin-grafted plants was achieved by higher Na+ exclusion in roots, which was driven by the Na+/H+ antiporter energized by the plasma membrane H+-ATPase, as evidenced by the higher plasma membrane H+-ATPase activity and higher transcript levels for PMA and SOS1. In addition, early stomatal closure was also observed in the pumpkin-grafted cucumber plants, reducing water loss and maintaining the plant’s hydration status. When pumpkin-grafted plants were pretreated with an NADPH oxidase inhibitor, diphenylene iodonium (DPI), the H2O2 level decreased significantly, to the level found in self-grafted plants, resulting in the loss of the salt tolerance. Inhibition of the NADPH oxidase-mediated H2O2 signaling in the root also abolished a rapid stomatal closure in the pumpkin-grafted plants. We concluded that the pumpkin-grafted cucumber plants increase their salt tolerance via a mechanism involving the root-sourced respiratory burst oxidase homologue-dependent H2O2 production, which enhances Na+ exclusion from the root and promotes an early stomatal closure.
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影响因子：
6.9
作者：
Chakraborty K;Bose J;Shabala L;Shabala S
通讯作者：
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影响因子：
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作者：
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影响因子：
6.9
作者：
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通讯作者：
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