Characterization of high-molecular-weight glutenin subunits from Eremopyrum bonaepartis and identification of a novel variant with unusual high molecular weight and altered cysteine residues
Characterization of high-molecular-weight glutenin subunits from Eremopyrum bonaepartis and identification of a novel variant with unusual high molecular weight and altered cysteine residues
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来自 Eremopyrum bonaepartis 的高分子量麦谷蛋白亚基的表征以及具有异常高分子量和改变的半胱氨酸残基的新变体的鉴定
DOI：
10.1007/s00425-013-2021-y
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发表时间：
2014-01
期刊：
影响因子：
4.3
通讯作者：
Wei YM
中科院分区：
文献类型：
--
作者：
Jiang QT;Zhang XW;Ma J;Wei L;Zhao S;Zhao QZ;Qi PF;Lu ZX;Zheng YL;Wei YM
我们鉴定了两个高分子量谷蛋白亚基(HMW-GS)的变异体，确定了它们的完整的开放阅读框，并在细菌系统中进行了表达。与Glu-1基因座编码的典型亚基相比，1Fx3.7和1Fy1.5具有许多新的结构特征：1Fx3.7和1Fy1.5表现出x型和y型亚基的杂交特性。此外，骨肉芽孢杆菌Glu-F1编码的HMW-GSS具有分子质量异常、半胱氨酸残基数量和分布发生变化等特点。成熟的1Fx3.7亚基全长1,223个氨基酸残基，是迄今发现的最大的亚基，而1Fy1.5只有496个残基。此外，在1Fx3.7的重复区还发现了突变的PGQQ重复基序。虽然它的分子质量与先前报道的1Dx2.2、1Dx2.2*和1Sshx2.9亚基相似，但1Fx3.7似乎有不同的进化史。N-末端和重复区域共有4个额外的半胱氨酸残基，1Fx3.7总共有8个半胱氨酸，而1Fy1.5只有6个半胱氨酸，因为重复区域末端的GHCPTSPQQ非肽被删除。由于1Fx3.7亚基含有额外的半胱氨酸残基和与优质小麦品质相关的最长重复区域，因此有可能成为小麦品质改良的理想候选基因。
We characterized two high-molecular-weight glutenin subunit (HMW-GS) variants from Eremopyrum bonaepartis, determined their complete open reading frames, and further expressed them in a bacterial system. The variants have many novel structural features compared with typical subunits encoded by Glu-1 loci: 1Fx3.7 and 1Fy1.5 exhibit hybrid properties of x- and y-type subunits. In addition, unusual molecular mass and altered number and distribution of cysteine residues were unique features of HMW-GSs encoded by Glu-F1 from E. bonaepartis. The mature 1Fx3.7 subunit has a full length of 1,223 amino acid residues, making it the largest subunit found thus far, while 1Fy1.5 is just 496 residues. In addition, the mutated PGQQ repeat motif was found in the repetitive region of 1Fx3.7. Although it has a similar molecular mass to that previously reported for 1Dx2.2, 1Dx2.2* and 1Sshx2.9 subunits, 1Fx3.7 appears to have had a different evolutionary history. The N-terminal and repetitive regions have a total of four additional cysteine residues, giving 1Fx3.7 a total of eight cysteines, while 1Fy1.5 has only six cysteines because the GHCPTSPQQ nonapeptide at the end of the repetitive region is deleted. With its extra cysteine residues and the longest repetitive region, features that are relevant to good wheat quality, the 1Fx3.7 subunit gene could be an excellent candidate for applications in wheat quality improvement.
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影响因子：
5.4
作者：
Y. Wan;Zehong Yan;Kunfan Liu;Youliang Zheng;R. D'Ovidio;P. Shewry;Nigel G Halford;Daowen Wang
通讯作者：
Y. Wan;Zehong Yan;Kunfan Liu;Youliang Zheng;R. D'Ovidio;P. Shewry;Nigel G Halford;Daowen Wang
影响因子：
5.4
作者：
LAWRENCE, GJ;SHEPHERD, KW
通讯作者：
SHEPHERD, KW
影响因子：
2.9
作者：
Feeney, KA;Wellner, N;Belton, PS
通讯作者：
Belton, PS
影响因子：
14.9
作者：
FORDE, J;MALPICA, JM;MIFLIN, BJ
通讯作者：
MIFLIN, BJ
影响因子：
5.4
作者：
Liu, Z.;Yan, Z.;Wang, D.
通讯作者：
Wang, D.