Brassinosteroid regulates stomatal development by GSK3-mediated inhibition of a MAPK pathway.
Brassinosteroid regulates stomatal development by GSK3-mediated inhibition of a MAPK pathway.
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DOI：
10.1038/nature10794
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发表时间：
2012-02-05
期刊：
影响因子：
64.8
通讯作者：
Wang, Zhi-Yong
中科院分区：
文献类型：
--
作者：
Kim, Tae-Wuk;Michniewicz, Marta;Bergmann, Dominique C.;Wang, Zhi-Yong
植物必须协调调节生化和解剖结构，以优化光合作用和水分利用效率。气孔的形成，即促进气体交换的表皮气孔，与光合作用发展的其他方面高度协调。然而，控制气孔发育的信号通路尚不完全清楚，尽管丝裂原活化蛋白激酶(MAPK)信号被认为在气孔发育中起关键作用。在这里，我们证明了油菜素类固醇(BR)通过激活MAPKKK，Yoda来调节气孔发育。遗传分析表明，受体激酶介导的BR信号通过GSK3样激酶BIN2抑制发育，BIN2作用于Yoda上游，但位于已知的ERECTA气孔受体激酶家族的下游。体外和体内的互补分析表明，BIN2使Yoda磷酸化以抑制其底物MKK4的Yoda磷酸化，在BR缺乏的突变体中，下游MAPKs的活性降低，但经BR或GSK3-激酶抑制剂处理后活性增加。我们的结果表明，BR通过减轻GSK3介导的对MAPK模块的抑制来抑制气孔发育，提供了两个关键环节：植物MAPKKK与其上游调节因子的联系以及BR与特定发育输出的联系。
Plants must coordinately regulate biochemistry and anatomy to optimize photosynthesis and water use efficiency. The formation of stomata, epidermal pores facilitating gas exchange, is highly coordinated with other aspects of photosynthetic development. However, the signaling pathways controlling stomata development are not fully understood, although Mitogen Activated Protein Kinase (MAPK) signaling is known to play key roles. Here we demonstrate that brassinosteroid (BR) regulates stomatal development by activating the MAPKKK, YODA. Genetic analyses indicate that receptor kinase-mediated BR signaling inhibits development through the GSK3-like kinase BIN2, and BIN2 acts upstream of YODA but downstream of the known ERECTA family of stomatal receptor kinases. Complementary in vitro and in vivo assays show that BIN2 phosphorylates YODA to inhibit YODA phosphorylation of its substrate MKK4, and activities of downstream MAPKs are reduced in BR-deficient mutants but increased by treatment with either BR or GSK3-kinase inhibitor. Our results indicate that BR inhibits stomatal development by alleviating GSK3-mediated inhibition of the MAPK module, providing two key links; that of a plant MAPKKK to its upstream regulators and BR to a specific developmental output.
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影响因子：
64.8
作者：
She, Ji;Han, Zhifu;Kim, Tae-Wuk;Wang, Jinjing;Cheng, Wei;Chang, Junbiao;Shi, Shuai;Wang, Jiawei;Yang, Maojun;Wang, Zhi-Yong;Chai, Jijie
通讯作者：
Chai, Jijie
DOI：
10.1111/j.1365-313x.2010.04145.x
发表时间：
2010-04
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
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通讯作者：
Jonak C
影响因子：
64.8
作者：
Chinchilla, Delphine;Zipfel, Cyril;Boller, Thomas
通讯作者：
Boller, Thomas
影响因子：
11.6
作者：
Wang, Huachun;Ngwenyama, Njabulo;Zhang, Shuqun
通讯作者：
Zhang, Shuqun
影响因子：
56.9
作者：
Li, JM;Nagpal, P;Chory, J
通讯作者：
Chory, J