Identification and characterization of six novel tetraspanins from Schistosoma japonicum.
Identification and characterization of six novel tetraspanins from Schistosoma japonicum.
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DOI：
10.1186/1756-3305-4-190
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发表时间：
2011-09-29
影响因子：
3.2
通讯作者：
Pan W
中科院分区：
文献类型：
--
作者：
Jiang Y;Xu X;Qing X;Pan W
四跨膜蛋白（TSP），也称为跨膜4超家族（TM 4SF）的成员，包含真核生物中报道的表面抗原的集合。在本研究中，从人血吸虫日本血吸虫（S.通过生物信息学和实验方法的组合产生和分析。6个日本血吸虫TSP新蛋白（Sj-TSP-#1~6）均含有4个跨膜区和1个大的胞外环（LEL），并具有保守的CCG基序。蛋白质的大小从227到291个氨基酸残基不等。所有六种蛋白质均在大肠杆菌中产生，并制备针对每种蛋白质的免疫血清。通过RT-PCR分析蛋白质的转录谱表明，Sj-TSP-#4仅在卵期转录，而Sj-TSP-#2在雌蠕虫中检测到转录，而在雄虫中未检测到。Western blot检测结果与此相似。免疫荧光检测表明，Sj-TSP-#2、Sj-TSP-#5和Sj-TSP-#6均定位于蠕虫体皮层。本研究提供了六个新的S.包括它们的序列和重组蛋白。新蛋白的可用性及其表达谱和位置的信息为进一步研究TSP蛋白的生物学功能和作为疫苗候选物提供了基础。
Tetraspanins (TSPs), also known as members of the trans-membrane 4 super-family (TM4SF), comprise an assemblage of surface antigens reported in eukaryotic organisms. In the work presented here, six novel TSP proteins from the human blood fluke Schistosoma japonicum (S. japonicum) were produced and analyzed through a combination of bioinformatics and experimental approaches. Six novel TSP proteins of Schistosoma japonicum (designated as Sj-TSP-#1~6) contained four trans-membrane regions and one large extracellular loop (LEL) with a conserved CCG motif. Size of the proteins varied from 227 to 291 amino acid residues. All the six proteins were produced in E.coli and immune sera to each protein were prepared. Analysis of transcription profiles of the proteins by RT-PCR showed that Sj-TSP-#4 was transcribed only in the egg stage while transcription of the Sj-TSP-#2 was detected in female worms but not in males. The similar results were obtained by Western blot. Immunolocalization of the TSP proteins by immunofluorescence assay showed that the Sj-TSP-#2, Sj-TSP-#5 and Sj-TSP-#6 were located in the tegument of worms. This study provided six novel TSP members of S. japonicum including their sequences and recombinant proteins. Availability of the novel proteins and information on their expression profile and location provided a basis for further investigation of the TSP proteins for their biological functions and as vaccine candidates.
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影响因子：
3.2
作者：
Driguez P;Doolan DL;Loukas A;Felgner PL;McManus DP
通讯作者：
McManus DP
影响因子：
3.2
作者：
Peng J;Han H;Gobert GN;Hong Y;Jiang W;Wang X;Fu Z;Liu J;Shi Y;Lin J
通讯作者：
Lin J
影响因子：
1.9
作者：
Glazar, Amanda I.;Evans, Janice P.
通讯作者：
Evans, Janice P.
影响因子：
4
作者：
Moribe, H;Yochem, J;Mekada, E
通讯作者：
Mekada, E
影响因子：
3.7
作者：
Garcia-Espana, Antonio;Mares, Roso;DeSalle, Rob
通讯作者：
DeSalle, Rob