Differential gene expression in Schistosoma japonicum schistosomula from Wistar rats and BALB/c mice.
Differential gene expression in Schistosoma japonicum schistosomula from Wistar rats and BALB/c mice.
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DOI：
10.1186/1756-3305-4-155
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发表时间：
2011-08-05
影响因子：
3.2
通讯作者：
Lin J
中科院分区：
文献类型：
--
作者：
Peng J;Han H;Gobert GN;Hong Y;Jiang W;Wang X;Fu Z;Liu J;Shi Y;Lin J
在中国大陆，超过46种哺乳动物可自然感染日本血吸虫。小鼠是宽容的，可能是生命周期的最终宿主。相比之下，大鼠对日本血吸虫感染的敏感性较低，并且被认为为寄生虫的生长和发育提供了不合适的微环境。由于我们对这种微环境对寄生虫本身的影响知之甚少，因此我们在本研究中利用日本血吸虫寡核苷酸微阵列来比较 Wistar 大鼠中维持的 10 日龄血吸虫与 BALB/c 小鼠中维持的基因表达差异。与 BALB/c 小鼠相比，Wistar 大鼠血吸虫基因中总共发现 3,468 个血吸虫基因存在差异表达，其中大多数（3,335 个）下调（≤ 2 倍），133 个上调（≥ 2 倍）。基因本体（GO）分析表明，在已确定功能或与另一种生物体中已明确表征的基因具有密切同源性的差异表达基因中，许多与重要的生物功能或分子过程有关。 Wistar 大鼠血吸虫中下调的基因中，一些与代谢、信号转导和发育有关。在这些与代谢过程相关的基因中，包括翻译、蛋白质和氨基酸磷酸化、蛋白水解、氧化还原酶活性、催化活性和水解酶活性等领域。差异表达基因的KEGG（京都基因和基因组百科全书）通路分析表明，在具有特定KEGG通路注释的328个基因中，有324个基因下调，主要与代谢、生长、氧化还原通路、氧化磷酸化、细胞周期、泛素介导的蛋白水解、蛋白输出和MAPK（丝裂原激活蛋白激酶）信号通路相关。这项工作提出了第一个大规模基因表达研究，确定了小鼠中维持的血吸虫与大鼠中维持的血吸虫之间的差异，并特别强调了可能影响最终宿主内寄生虫的生存和发育的差异表达。这里介绍的研究为更好地了解血吸虫发育和宿主-寄生虫相互作用提供了有价值的信息。
More than 46 species of mammals can be naturally infected with Schistosoma japonicum in the mainland of China. Mice are permissive and may act as the definitive host of the life cycle. In contrast, rats are less susceptible to S. japonicum infection, and are considered to provide an unsuitable micro-environment for parasite growth and development. Since little is known of what effects this micro-environment has on the parasite itself, we have in the present study utilised a S. japonicum oligonucleotide microarray to compare the gene expression differences of 10-day-old schistosomula maintained in Wistar rats with those maintained in BALB/c mice. In total 3,468 schistosome genes were found to be differentially expressed, of which the majority (3,335) were down-regulated (≤ 2 fold) and 133 were up-regulated (≥ 2 fold) in schistosomula from Wistar rats compared with those from BALB/c mice. Gene ontology (GO) analysis revealed that of the differentially expressed genes with already established functions or close homology to well characterized genes in another organisms, many are related to important biological functions or molecular processes. Among the genes that were down-regulated in schistosomula from Wistar rats, some were associated with metabolism, signal transduction and development. Of these genes related to metabolic processes, areas including translation, protein and amino acid phosphorylation, proteolysis, oxidoreductase activities, catalytic activities and hydrolase activities, were represented. KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analysis of differential expressed genes indicated that of the 328 genes that had a specific KEGG pathway annotation, 324 were down-regulated and were mainly associated with metabolism, growth, redox pathway, oxidative phosphorylation, the cell cycle, ubiquitin-mediated proteolysis, protein export and the MAPK (mitogen-activated protein kinases) signaling pathway. This work presents the first large scale gene expression study identifying the differences between schistosomula maintained in mice and those maintained in rats, and specifically highlights differential expression that may impact on the survival and development of the parasite within the definitive host. The research presented here provides valuable information for the better understanding of schistosome development and host-parasite interactions.
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