Functionalized magnetic microparticle-based colorimetric platform for influenza A virus detection.
Functionalized magnetic microparticle-based colorimetric platform for influenza A virus detection.
复制标题
用于甲型流感病毒检测的功能化磁性微粒比色平台
DOI：
10.1088/0957-4484/27/43/435102
复制
发表时间：
2016-10-28
期刊：
影响因子：
3.5
通讯作者：
He Z
中科院分区：
文献类型：
--
作者：
Chen C;Zou Z;Chen L;Ji X;He Z
利用酶催化的高效性和过氧化氢还原金离子的特性，建立了一种检测甲型流感病毒的比色平台。合成了适体功能化的磁性微粒以捕获甲型流感病毒。随后是ConA-GOx-AuNP通过ConA-聚糖相互作用与H3 N2病毒的结合。随后将夹心复合物分散在葡萄糖溶液中以引发酶促反应产生过氧化氢，过氧化氢控制金纳米颗粒的生长并产生有色溶液。通过比较两种不同颜色的金纳米颗粒实现了H3 N2浓度的测定。该方法可以检测到低至11.16 μg ml−1的目标病毒。此外，它为病毒和蛋白质的灵敏和比色检测开辟了新的机会。
A colorimetric platform for influenza A virus detection was developed by using the high efficiency of enzymatic catalysis and the reduction of gold ions with hydrogen peroxide. Aptamer-functionalized magnetic microparticles were synthesized to capture the influenza A virus. This was followed by the binding of ConA-GOx-AuNPs to the H3N2 virus through the ConA-glycan interaction. The sandwich complex was subsequently dispersed in glucose solution to trigger an enzymatic reaction to produce hydrogen peroxide, which controlled the growth of gold nanoparticles and produced colored solutions. The determination of H3N2 concentration was realized by comparing the two differently colored gold nanoparticles. This method could detect the target virus as low as 11.16 μg ml−1. Furthermore, it opens new opportunities for sensitive and colorimetric detection of viruses and proteins.
登录
查看更多内容
影响因子：
88.1
作者：
Medina, Rafael A.;Garcia-Sastre, Adolfo
通讯作者：
Garcia-Sastre, Adolfo
影响因子：
3.1
作者：
Lee, MS;Chang, PC;Shieh, HK
通讯作者：
Shieh, HK
影响因子：
9.4
作者：
Spackman, E;Senne, DA;Suarez, DL
通讯作者：
Suarez, DL
影响因子：
7.4
作者：
GRABAR, KC;FREEMAN, RG;NATAN, MJ
通讯作者：
NATAN, MJ
影响因子：
56.9
作者：
Russell, Colin A.;Jones, Terry C.;Smith, Derek J.
通讯作者：
Smith, Derek J.