Elucidation of the Molecular Mechanisms of Action of the Natural Antimicrobial Peptide Subtilosin Against the Bacterial Vaginosis-associated Pathogen Gardnerella vaginalis.
Elucidation of the Molecular Mechanisms of Action of the Natural Antimicrobial Peptide Subtilosin Against the Bacterial Vaginosis-associated Pathogen Gardnerella vaginalis.
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DOI：
10.1007/s12602-010-9061-4
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发表时间：
2011-03
影响因子：
4.9
通讯作者：
Chikindas, Michael L.
中科院分区：
文献类型：
--
作者：
Noll, Katia Sutyak;Sinko, Patrick J.;Chikindas, Michael L.
枯草杆菌素A是由解淀粉芽孢杆菌产生的35个氨基酸长的环状肽，其对多种人类病原体具有有效的抗微生物活性，包括细菌性阴道病相关的阴道加德纳菌。枯草杆菌素对G.通过研究迷走神经对质子动力（PMF）的跨膜电位（Δ P）、跨膜pH梯度（ΔpH）和细胞内ATP水平的影响，阐明了迷走神经的作用机制。在G.迷走神经细胞引起ΔpH的立即和完全耗尽，但对Δ pH没有影响。枯草杆菌素也触发了瞬间但部分流出的细胞内ATP，是两倍高于阳性对照细菌素，乳酸链球菌素。综上所述，这些数据表明subtilosin抑制G。通过在细胞的细胞质膜中产生瞬时孔，导致细胞内离子和ATP流出并最终导致细胞死亡，从而导致迷走神经生长。
Subtilosin A is a 35-amino acid long cyclical peptide produced by Bacillus amyloliquefaciens that has potent antimicrobial activity against a variety of human pathogens, including the bacterial vaginosis-related Gardnerella vaginalis. The specific mode of action of subtilosin against G. vaginalis was elucidated by studying its effects on the proton motive force’s (PMF) components: transmembrane electric potential (ΔΨ), transmembrane pH gradient (ΔpH), and intracellular ATP levels. The addition of subtilosin to G. vaginalis cells caused an immediate and total depletion of the ΔpH, but had no effect on the ΔΨ. Subtilosin also triggered an instant but partial efflux of intracellular ATP that was twofold higher than that of the positive control bacteriocin, nisin. Taken together, these data suggest that subtilosin inhibits G. vaginalis growth by creating transient pores in the cells’ cytoplasmic membrane, leading to an efflux of intracellular ions and ATP and eventually cell death.
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DOI：
10.1155/s1064744999000277
发表时间：
1999-01-01
影响因子：
--
作者：
Lin, L;Song, J;Faro, S
通讯作者：
Faro, S
影响因子：
11.8
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
158.5
作者：
Hillier, SL;Nugent, RP;Klebanoff, MA
通讯作者：
Klebanoff, MA
影响因子：
5.9
作者：
AMSEL, R;TOTTEN, PA;HOLMES, KK
通讯作者：
HOLMES, KK