Peptides from the Intestinal Tract of Breast Milk-Fed Infants Have Antimicrobial and Bifidogenic Activity.
Peptides from the Intestinal Tract of Breast Milk-Fed Infants Have Antimicrobial and Bifidogenic Activity.
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DOI：
10.3390/ijms22052377
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发表时间：
2021-02-27
影响因子：
5.6
通讯作者：
Dallas DC
中科院分区：
文献类型：
--
作者：
Beverly RL;Woonnimani P;Scottoline BP;Lueangsakulthai J;Dallas DC
对于与婴儿健康相关的生物活性乳肽，它们必须通过胃肠道蛋白水解释放，并抵抗进一步的蛋白水解，直到它们到达其活性位点。肠道是大多数生物活性最可能的部位，但目前尚不清楚其中是否存在生物活性乳肽。本研究的目的是鉴定婴儿肠道中的抗菌肽和双歧杆菌肽。从婴儿肠道样品中提取乳肽，通过测定大肠杆菌、金黄色葡萄球菌和长双歧杆菌的生长来测定散装肽提取物的活性。用系列稀释液孵育后的婴儿。通过肽组学分析测定活性和非活性样品的肽谱，并进行比较，以鉴定用于生物活性检测的候选肽。我们从16名婴儿的29份肠道样品中提取了肽。5个样品对S.金黄色葡萄球菌和6个样品具有双歧杆菌活性。婴儿。我们将6645个乳肽的列表缩小到11个用于合成的候选肽，其中6个完全抑制E. coli和革兰氏阳性菌S.浓度为2500和3000 µg/mL时的金黄色葡萄球菌生长。本研究为乳肽在婴儿肠道中的潜在生物活性提供了证据。
For bioactive milk peptides to be relevant to infant health, they must be released by gastrointestinal proteolysis and resist further proteolysis until they reach their site of activity. The intestinal tract is the likeliest site for most bioactivities, but it is currently unknown whether bioactive milk peptides are present therein. The purpose of the present study was to identify antimicrobial and bifidogenic peptides in the infant intestinal tract. Milk peptides were extracted from infant intestinal samples, and the activities of the bulk peptide extracts were determined by measuring growth of Escherichia coli, Staphylococcus aureus, and Bifidobacterium longum spp. infantis after incubation with serial dilutions. The peptide profiles of active and inactive samples were determined by peptidomics analysis and compared to identify candidate peptides for bioactivity testing. We extracted peptides from 29 intestinal samples collected from 16 infants. Five samples had antimicrobial activity against S. aureus and six samples had bifidogenic activity for B. infantis. We narrowed down a list of 6645 milk peptides to 11 candidate peptides for synthesis, of which 6 fully inhibited E. coli and S. aureus growth at concentrations of 2500 and 3000 µg/mL. This study provides evidence for the potential bioactivity of milk peptides in the infant intestinal tract.
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影响因子：
5.1
作者：
Donovan, Sharon M.
通讯作者：
Donovan, Sharon M.
影响因子：
3.6
作者：
Coppa, GV;Zampini, L;Orazio, G
通讯作者：
Orazio, G
影响因子：
5.1
作者：
Boghossian, Nansi S.;Page, Grier P.;Higgins, Rosemary D.
通讯作者：
Higgins, Rosemary D.
影响因子：
56.9
作者：
CARPENTER, G
通讯作者：
CARPENTER, G
影响因子：
15.9
作者：
ELLISON, RT;GIEHL, TJ
通讯作者：
GIEHL, TJ