Phosphate-solubilizing Bacteria from Safflower Rhizosphere and their Effect on Seedling Growth.

Phosphate-solubilizing Bacteria from Safflower Rhizosphere and their Effect on Seedling Growth.
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DOI:
10.1515/biol-2019-0028
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发表时间:
2019-01
期刊:
影响因子:
2.2
通讯作者:
Ma L
Ma L
中科院分区:
生物学4区
文献类型:
--
作者:
Zhang T;Hu F;Ma L

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解磷细菌(PSB)可以将根际不溶性磷转化为植物可吸收的形式,从而促进植物的生长。红花是一种经济作物,是植物油、食用色素和调味剂的来源。本研究旨在分离红花根际土壤中的光合细菌,并研究其对红花幼苗生长的影响。经16S rRNA基因序列分析,鉴定为假单胞菌属(Pseudomonas)、中华根瘤菌属(Sinorhizobium)、葡萄球菌属(Staphylococcus)、不动杆菌属(Acinetobacter)和肠杆菌属(Enterobacter)。不动杆菌属RC 04。表现出最好的性能,在磷的溶解,与该过程的效率受到碳源,氮源,培养温度和初始培养pH值的影响。不动杆菌属物种RC04和中华根瘤菌属物种RC02表现出提高红花种子发芽的能力,当共同接种,提高幼苗生长。因此,我们建议开发不动杆菌RC 04和中华根瘤菌RC 02用于促进红花生长的田间应用。这项研究的结果将有助于推动新的生物肥料的发现,并可能被纳入红花和其他重要经济作物的综合营养管理制度。
Phosphate-solubilizing bacteria (PSB) can convert insoluble rhizosphere phosphorus into forms that are absorbable by plants and thus enhance the growth of plants. Safflower is a cash crop that is a source of vegetable oils, food coloring and flavoring agents. This study sought to isolate PSB in safflower rhizosphere soil and investigate their effects on seedling growth. The isolated PSB were identified as belonging to the genera Pseudomonas, Sinorhizobium, Staphylococcus, Acinetobacter and Enterobacter using 16S rRNA gene sequence analysis. Acinetobacter sp RC04. showed the best performance in phosphate solubilization, with the efficiency of the process being influenced by carbon source, nitrogen source, cultivation temperature and initial culture pH. Acinetobacter sp. RC04 and Sinorhizobium sp. RC02 showed the ability to improve safflower seed germination and, when co-inoculated, improved seedling growth. Hence, we suggest that Acinetobacter sp. RC04 and Sinorhizobium sp. RC02 could be developed for field application to promote safflower growth. The results from this study will help drive novel biofertilizer discovery and could be included in integrated nutrient management regimes for safflower and other important economic crops.
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