Occurrence and cytological mechanism of numerically unreduced pollen in diploid Populus euphratica
Occurrence and cytological mechanism of numerically unreduced pollen in diploid Populus euphratica
复制标题
二倍体胡杨花粉数量未减少的发生及其细胞学机制
DOI：
10.1515/sg-2013-0034
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发表时间：
2013-12
期刊：
影响因子：
1
通讯作者：
Kang Xiangyang
中科院分区：
文献类型：
--
作者：
Zhang Pingdong;Kang Xiangyang
摘要未减数配子是自然界中植物多倍体化的驱动力，也是培育三倍体个体的重要工具。2n花粉粒最终的杂合性取决于2n花粉形成的细胞学机制。本研究利用荧光染色和间接免疫荧光技术对18个胡杨二倍体基因型的小孢子发生过程中减数分裂异常进行了分析。(2n= 2x = 38）。在18个基因型中，16个基因型产生2n花粉，2个基因型只产生正常n花粉。在所有的2N花粉生产者，我们发现，第一次减数分裂是正常的，但第二次分裂的特点是频繁异常纺锤体方向（平行，三极，融合纺锤体）和过早的胞质分裂。平行融合的纺锤体和过早的胞质分裂被认为是导致二分体形成的原因，三极纺锤体似乎是导致四分体形成的原因。平行纺锤体的出现频率高于其他纺锤体形式，但平行纺锤体与二分体的相关性不显著。对小孢子母细胞减数分裂Ⅱ的间接免疫荧光观察表明，平行纺锤体导致一个或两个非姐妹染色体组的聚集，导致两个子核的RMS的掺入。因此，整合的RMS建立了两个核质结构域，用于控制分裂平面，导致三联体或二联体的形成。
Abstract Unreduced gametes are the driving force for the polyploidizaiton of plants in nature, and are also an important tool for breeding of triploid individuals. The final heterozygosity of a 2n pollen grain depends on the cytological mechanism behind 2n pollen formation. In this study, meiotic abnormalities were analysed using fluorescent chromosome staining and indirect immunofluorescence during the microsporogenesis of 18 genotypes of diploid P. euphratica Oliv. (2n = 2x = 38). Among the 18 genotypes, 16 genotypes produce 2n pollen and two genotypes produce only normal n pollen. In all 2n pollen producers, we found that the first meiotic division was normal but that the second division was characterized by frequent abnormal spindle orientation (parallel, tripolar, and fused spindles) and premature cytokinesis. The parallel, fused spindles and premature cytokinesis were considered to be leading dyad formation, and tripolar spindles seemed to be causing triad formation at the tetrad stage. There was a higher frequency of parallel spindles than other spindle forms, but no significant correlations between parallel spindles and dyads were observed. However, a significant association (r = 0.68, P < 0.05) between the tripolar spindles and dyads was found. In some Microspore mother cells (MMCs), an indirect immunofluorescence examination of meiosis II revealed that the parallel spindles led to the gathering of one or two non-sister groups of chromosomes, causing an incorporation of RMSs from two daughter nuclei. Therefore, the incorporated RMSs established two nuclear cytoplasmic domains for the control of division plane, resulting in either triad or dyad formation.
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影响因子：
3.1
作者：
S. Peloquin;G. Yerk;J. E. Werner;E. Darmo
通讯作者：
S. Peloquin;G. Yerk;J. E. Werner;E. Darmo
影响因子：
3.3
作者：
S. J. Peloquin;L. S. Boiteux;Domenico Carputo
通讯作者：
S. J. Peloquin;L. S. Boiteux;Domenico Carputo
影响因子：
3.1
作者：
G. Genualdo;A. Errico;A. Tiezzi;C. Conicella
通讯作者：
G. Genualdo;A. Errico;A. Tiezzi;C. Conicella
DOI：
10.1139/g82-032
发表时间：
1982-06
期刊：
Canadian journal of genetics and cytology
影响因子：
--
作者：
R. Veilleux;N. McHale;F. Lauer
通讯作者：
R. Veilleux;N. McHale;F. Lauer
影响因子：
1.9
作者：
Zheng-hai Zhang;X. Kang
通讯作者：
Zheng-hai Zhang;X. Kang