Refractive index mapping of single cells with a graphene-based optical sensor
Refractive index mapping of single cells with a graphene-based optical sensor
复制标题
使用基于石墨烯的光学传感器对单细胞进行折射率映射
DOI：
10.1016/j.snb.2016.11.027
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发表时间：
2017-04
期刊：
影响因子：
--
通讯作者：
Xiaocong Yuan
中科院分区：
文献类型：
--
作者：
Lixun Sun;Yuquan Zhang Yijia Wang Chonglei Zhang Changjun;Xiaocong Yuan
基于石墨烯的光学传感器在电池折射率（RI）测量中表现出良好的性能，具有高分辨率和大探测深度。在这篇论文中，我们报道了一种在聚焦扫描模式下基于石墨烯偏振敏感光吸收的RI映射方法。已经获得了一些正常和癌变的人类结肠细胞的RI作图图像。一个高数值孔径显微镜物镜被用来最小化聚焦光斑的大小。在优化条件下，实验空间分辨率达到2.34 μm。生成了直径可调的环形完美光学涡旋光束，可控制入射角，实验RI分辨率为9.3 × 10−4折射率单位。石墨烯消失场的大纵向穿透深度允许识别两个单个癌细胞之间的RI差异。可以确定细胞的RI分布和形态，并从图像中推断不同的癌变阶段。这种方法在生物医学科学，特别是细胞成像、分化和分选方面具有潜力。
Graphene-based optical sensors exhibit good performance in cell refractive index (RI) measurements, with high resolution and large detection depths. In this manuscript, we report an RI mapping method based on the polarization-sensitive light absorption of graphene, in focused scanning mode. Several RI mapping images of normal and cancerous human colonic cells have been obtained. A high-numerical-aperture microscope objective is used to minimize the focusing spot size. Under optimized conditions, the experimental spatial resolution reaches 2.34 μm. A ring-shaped perfect optical vortex beam with a tunable diameter is generated to control the incident angles, and the experimental RI resolution is 9.3 × 10−4refractive index units. The large longitudinal penetration depth of the graphene evanescent field allows the recognition of RI difference between two individual cancer cells. The RI distribution and morphology of the cells can be determined, and different carcinogenesis stages can also be concluded from the image. This method has potential in biomedical sciences, especially in cell imaging, differentiation, and sorting.
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作者：
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通讯作者：
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