Effects of the tiletamine/zolazepam-xylazine-tramadol combination on plasma oxidative status and haematological indicators in miniature pigs
Effects of the tiletamine/zolazepam-xylazine-tramadol combination on plasma oxidative status and haematological indicators in miniature pigs
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替来他明/佐拉西泮-赛拉嗪-曲马多联合用药对小型猪血浆氧化状态和血液学指标的影响
DOI：
10.2754/avb201483020145
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发表时间：
2014-06
影响因子：
0.6
通讯作者：
Wang, Hong-Bin
中科院分区：
文献类型：
--
作者：
Hau, Jin-Long;Song, Xu-Dong;Wang, Yue;Wang, Hong-Bin
当自由基的产生破坏抗氧化防御系统时，体内的氧化应激就会发生。本研究的目的是检查替来他明/唑拉西泮-赛拉嗪-曲马多组合对8只小型猪的抗氧化状态、脂过氧化物酶和血细胞指标的影响。在麻醉前记录基线测量，然后用替来他明/唑拉西泮（3.5mg/kg）、甲苯噻嗪（1.32mg/kg）和曲马多（1.8mg/kg）的组合麻醉动物。分别于麻醉后15、30、60、90、120 min、24 h和72 h从前腔静脉采集血样。采用比色法测定血浆丙二醛、超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶浓度，采用自动细胞计数器测定红细胞计数、白色细胞计数、血红蛋白和红细胞压积。结果显示，注射后30、60 min丙二醛浓度显著升高（P0.05），90 min后恢复至基线水平（P > 0.05）。SOD活性显著升高红细胞计数、血红蛋白和红细胞压积变化不明显，而白色血细胞计数显著降低我们的研究首次证明了替来他明/唑拉西泮-赛拉嗪-曲马多提供抗氧化作用，可用于减轻兽医诊所检查和研究或长途运输的压力。
Oxidative stress in the body occurs when the production of free radicals overwhelms the antioxidant defence systems. The aim of this study was to examine the effects of a tiletamine/ zolazepam-xylazine-tramadol combination on the antioxidant status, lipoperoxidase and blood cell indicators in eight miniature pigs. Baseline measures were recorded prior to anaesthesia, then the animals were anaesthetized with the combination of tiletamine/zolazepam (3.5 mg/kg), xylazine (1.32 mg/kg), and tramadol (1.8 mg/kg). Blood samples were collected from the anterior vena cava at 15, 30, 60, 90, 120 min, 24 h, and 72 h after anaesthesia. Plasma malondialdehyde, superoxide dismutase, catalase, and glutathione peroxidase concentrations were measured by colorimetry, and red blood cell counts, white blood cell counts, haemoglobin and packed cell volume were determined using an automated cell counter. The results showed that the concentration of malondialdehyde increased significantly at 30 and 60 min after the injection (P 0.05) then returned to baseline values after 90 min (P > 0.05). Superoxide dismutase activity increased significantly (P < 0.05) at 30 and 60 min, and then gradually decreased to baseline values after 90 min. Changes in red blood cell counts, haemoglobin and packed cell volume were not significant, while white blood cell count decreased significantly (P < 0.05) at 30 and 60 min. Our study is the first to demonstrate that tiletamine/zolazepam-xylazine-tramadol provide antioxidant effects, which may be proposed for alleviating the stress of examination and research at veterinary clinics or long-distance transportation.
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影响因子：
2.9
作者：
Y. Ōyanagui
通讯作者：
Y. Ōyanagui
影响因子：
6.1
作者：
B. Halliwell
通讯作者：
B. Halliwell
影响因子：
2.2
作者：
K. Singh;P. Kinjavdekar;Amarpal;H. Aithal;A. Gopinathan;G. Singh;A. Pawde;K. Pratap
通讯作者：
K. Singh;P. Kinjavdekar;Amarpal;H. Aithal;A. Gopinathan;G. Singh;A. Pawde;K. Pratap
影响因子：
1
作者：
DeRossi, R;Junqueira, AL;Beretta, MP
通讯作者：
Beretta, MP
DOI：
10.2460/javma.2002.220.1491
发表时间：
2002-05
期刊：
Journal of the American Veterinary Medical Association
影响因子：
--
作者：
L. S. Williams;J. Levy;S. Robertson;A. M. Cistola;L. A. Centonze
通讯作者：
L. S. Williams;J. Levy;S. Robertson;A. M. Cistola;L. A. Centonze