Comparison among nasopharyngeal swab, nasal wash, and oropharyngeal swab for respiratory virus detection in adults with acute pharyngitis.
Comparison among nasopharyngeal swab, nasal wash, and oropharyngeal swab for respiratory virus detection in adults with acute pharyngitis.
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DOI：
10.1186/1471-2334-13-281
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发表时间：
2013-06-20
影响因子：
3.7
通讯作者：
Zhong NS
中科院分区：
文献类型：
--
作者：
Li L;Chen QY;Li YY;Wang YF;Yang ZF;Zhong NS
急性咽炎是常见的初级保健。急性病毒性咽炎易误诊为急性细菌性咽炎。建议对呼吸道病毒进行经检验确认的诊断。本研究旨在比较口咽拭子（OPS）、鼻咽拭子（NEP）和鼻洗液（NW）对成人急性咽炎的敏感性。OPS、OLP和NW均来自急性咽炎患者。采用TaqMan实时定量聚合酶链反应检测15种呼吸道病毒。如果任何标本呈阳性，则认为该样本为真阳性。通过卡方检验或Fisher精确检验（如适用）比较样本间的灵敏度。获得了OPS、OLS和NW连续采集的103个三重样品。在73例患者中，通过三种方法中的任何一种检测到一种或多种病毒。在所有病毒中，ELISA的敏感性显著高于NW（分别为74%对49%; p < 0.01）和OPS（分别为74%对49%; p < 0.01）。使用TaqMan实时聚合酶链反应检测成人急性咽炎呼吸道病毒，群集式病毒采集可能是NW和OPS的最有效替代方法。
Acute pharyngitis is frequently seen in primary care. Acute viral pharyngitis may be easily misdiagnosed as acute bacterial pharyngitis. Laboratory-confirmed diagnosis of respiratory viruses is recommended. The purpose of this study was to compare the sensitivities among oropharyngeal swab (OPS), nasopharyngeal swab (NPS), and nasal wash (NW) in adults with acute pharyngitis. OPS, NPS, and NW were obtained from each participant with acute pharyngitis. The specimens were tested for 15 respiratory viruses by TaqMan real-time polymerase chain reaction. A sample was considered to be a true positive if any of the specimens was positive. The sensitivities among samples were compared by chi-square test or Fisher’s exact test, as appropriate. One hundred three triple samples collected consecutively by OPS, NPS, and NW were obtained. In 73 patients, one or more viruses were detected by any of the three methods. Among all viruses, the sensitivity of NPS was significantly higher than that of NW (74% vs. 49%, respectively; p < 0.01) and OPS (74% vs. 49%, respectively; p < 0.01). Flocked NPS collection may be the most effective alternative to NW and OPS for detection of respiratory viruses in adults with acute pharyngitis using TaqMan real-time polymerase chain reaction.
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