Mucin gene expression and mouse middle ear epithelium.
Mucin gene expression and mouse middle ear epithelium.
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DOI：
10.1016/j.ijporl.2010.04.014
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发表时间：
2010-08
影响因子：
1.5
通讯作者：
Khampang, P.
中科院分区：
文献类型：
--
作者：
Kerschner, Joseph E.;Li, Jizhen;Tsushiya, Katsuyuki;Khampang, P.
研究最近鉴定的人粘蛋白基因在体外培养的小鼠中耳上皮细胞（MMEEC）模型中的表达。建立MMEEC，提取RNA，设计引物进行RT-PCR检测Muc 1、Muc 2、Muc 3、Muc 4、Muc 5AC、Muc 5 B、Muc 6、Muc 7、Muc 8、Muc 9、Muc 10、Muc 11/12、Muc 13、Muc 15、Muc 16、Muc 17、Muc 18、Muc 19和Muc 20的表达。在MMEEC中鉴定并表达粘蛋白基因Muc 1、Muc 2、Muc 3、Muc 4、Muc 5AC、Muc 5 B、Muc 9、Muc 10、Muc 13、Muc 15、Muc 16、Muc 18、Muc 19和Muc 20。未鉴定出Muc 6、Muc 7、Muc 8、Muc 11/12和Muc 17基因。许多最近在人类大肠杆菌和灰鼠大肠杆菌中鉴定的粘蛋白基因也在MMEEC中表达。然而，表达存在差异，这可能对利用各种动物模型研究中耳生理学和发病机制具有影响;特别是因为它涉及粘蛋白基因表达。
To investigate the expression of recently identified human mucin genes in an in vitro model of cultured mouse middle ear epithelial cells (MMEEC). MMEEC were established, RNA was extracted and primers were designed for RT-PCR to assess for expression of mucin genes Muc1, Muc2, Muc3, Muc4, Muc5AC, Muc5B, Muc6, Muc7, Muc8, Muc9, Muc10, Muc11/12, Muc13, Muc15, Muc16, Muc17, Muc18, Muc19 and Muc20 expression. Mucin genes Muc1, Muc2, Muc3, Muc4, Muc5AC, Muc5B, Muc9, Muc10, Muc13, Muc15, Muc16, Muc18, Muc19 and Muc20 were identified and expressed in MMEEC. The genes Muc6, Muc7, Muc8, Muc11/12 and Muc17 were not identified. Many of the mucin genes that have been recently identified in human MEE and chinchilla MEE are also expressed in MMEEC. There are differences in expression, however, which may have implications in utilizing various animal models for study of middle ear physiology and pathogenesis; specifically as it relates to mucin gene expression.
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影响因子：
1.4
作者：
Lee, HM;Kim, DH;Hwang, SJ
通讯作者：
Hwang, SJ
DOI：
10.1073/pnas.0705984104
发表时间：
2007-10-09
影响因子：
11.1
作者：
Lang, Tiange;Hansson, Gunnar C.;Samuelsson, Tore
通讯作者：
Samuelsson, Tore
DOI：
10.1152/ajpgi.00491.2005
发表时间：
2006-08-01
影响因子：
4.5
作者：
Malmberg, Emily K.;Noaksson, Karin A.;Hansson, Gunnar C.
通讯作者：
Hansson, Gunnar C.
影响因子：
6.4
作者：
Caramori, Gaetano;Casolari, Paolo;Papi, Alberto
通讯作者：
Papi, Alberto
影响因子：
4.8
作者：
Shuto, T;Imasato, A;Li, JD
通讯作者：
Li, JD