Lytic and Chemotactic Features of the Plaque-Forming Bacterium KD531 on Phaeodactylum tricornutum.
Lytic and Chemotactic Features of the Plaque-Forming Bacterium KD531 on Phaeodactylum tricornutum.
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三角褐指菌上噬菌斑形成细菌 KD531 的裂解和趋化特性
DOI：
10.3389/fmicb.2017.02581
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发表时间：
2017
影响因子：
5.2
通讯作者：
Xu H
中科院分区：
文献类型：
--
作者：
Chen Z;Zheng W;Yang L;Boughner LA;Tian Y;Zheng T;Xu H
三角褐指藻是水华形成的优势种，也是潜在的生物燃料原料。为了控制三角链霉菌的水华或从三角链霉菌中释放脂类，我们先前筛选和鉴定了裂解细菌Labrenzia sp.KD531，朝向三角褐指藻。在本研究中，我们评价了Labrenzia sp.的裂解活性。KD531对微藻的裂解作用，并探讨其裂解机理。结果表明，KD531的裂解活性依赖于温度和pH，但不依赖于光。除三角褐指藻外，KD531对其他藻类，尤其是绿藻也有一定的溶藻活性。通过对藻细胞蛋白质、碳水化合物和脂肪含量的定量分析，以及接触细菌感染的藻裂解液后干重的测定，表明细菌KD531通过破坏藻细胞而影响藻类的生物量。趋化分析和斑块形成不同区域的亚样的显微镜观察都表明，KD531可以向藻细胞移动，然后直接与藻细胞接触。三角假单胞菌与KD531细胞之间的直接接触是裂解过程所必需的。
Phaeodactylum tricornutum is a dominant bloom forming species and potential biofuel feedstock. To control P. tricornutum bloom or to release lipids from P. tricornutum, we previously screened and identified the lytic bacterium Labrenzia sp. KD531 toward P. tricornutum. In the present study, we evaluated the lytic activity of Labrenzia sp. KD531 on microalgae and investigated its lytic mechanism. The results indicated that the lytic activity of KD531 was temperature- and pH-dependent, but light-independent. In addition to P. tricornutum, KD531 also showed lytic activity against other algal species, especially green algae. A quantitative analysis of algal cellular protein, carbohydrate and lipid content together with measurements of dry weight after exposure to bacteria-infected algal lysate indicated that the bacterium KD531 influenced the algal biomass by disrupting the algal cells. Both chemotactic analysis and microscopic observations of subsamples from different regions of formed plaques showed that KD531 could move toward and then directly contact algal cells. Direct contact between P. tricornutum and KD531 cells was essential for the lytic process.
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影响因子：
5.6
作者：
GERBER, BR;ASAKURA, S;OOSAWA, F
通讯作者：
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影响因子：
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作者：
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影响因子：
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作者：
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影响因子：
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作者：
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影响因子：
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作者：
Li Y;Lei X;Zhu H;Zhang H;Guan C;Chen Z;Zheng W;Fu L;Zheng T
通讯作者：
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