Regions of XY homology in the pig X chromosome and the boundary of the pseudoautosomal region.
Regions of XY homology in the pig X chromosome and the boundary of the pseudoautosomal region.
复制标题
DOI：
10.1186/1471-2156-14-3
复制
发表时间：
2013-01-15
期刊：
影响因子：
2.9
通讯作者：
Affara NA
中科院分区：
文献类型：
--
作者：
Skinner BM;Lachani K;Sargent CA;Affara NA
性染色体受到不同于基因组其余部分的进化压力，塑造它们的结构和序列内容。我们感兴趣的是家猪（Sus scrofa）的性染色体，它们的结构和基因内容如何与其他哺乳动物物种进行比较和对比，以及性连锁基因在生育中的作用。这需要了解这些染色体上的XY同源序列。为此，我们进行了基于微阵列的比较基因组杂交（阵列CGH）与男性和女性杜洛克猪的X染色体BAC平铺路径微阵列的基因组DNA。随后对来自感兴趣区域的推定XY同源BAC进行FISH作图。我们发现，猪PAR约为6.5-6.9 Mb的开始的短臂的X，与偶蹄动物共同祖先的基因内容反映。我们的阵列CGH数据还显示了靠近X长臂末端的XY同源区域，跨越三个X BAC。这些BAC是FISH映射的，并绘制了SSCY的整个长臂。进一步克隆的兴趣揭示了X-常染色体同源性或含有重复内容的区域。本研究确定了猪基因组中XY同源区域，并确定了X染色体上PAR的边界。这增加了我们对性染色体在不同哺乳动物谱系中进化的理解，并将证明对未来suids的比较基因组工作以及性染色体基因组序列的构建和注释有价值。我们发现SSCYq重复内容在X染色体上有相应的序列，这进一步深入了解了SSCY的结构，并表明在X和Y上仍有待发现更多功能重要的序列。
Sex chromosomes are subject to evolutionary pressures distinct from the remainder of the genome, shaping their structure and sequence content. We are interested in the sex chromosomes of domestic pigs (Sus scrofa), how their structure and gene content compares and contrasts with other mammalian species, and the role of sex-linked genes in fertility. This requires an understanding of the XY-homologous sequence on these chromosomes. To this end, we performed microarray-based comparative genomic hybridisation (array-CGH) with male and female Duroc genomic DNA on a pig X-chromosome BAC tiling-path microarray. Putative XY-homologous BACs from regions of interest were subsequently FISH mapped. We show that the porcine PAR is approximately 6.5-6.9 Mb at the beginning of the short arm of the X, with gene content reflective of the artiodactyl common ancestor. Our array-CGH data also shows an XY-homologous region close to the end of the X long arm, spanning three X BACs. These BACs were FISH mapped, and paint the entire long arm of SSCY. Further clones of interest revealed X-autosomal homology or regions containing repetitive content. This study has identified regions of XY homology in the pig genome, and defined the boundary of the PAR on the X chromosome. This adds to our understanding of the evolution of the sex chromosomes in different mammalian lineages, and will prove valuable for future comparative genomic work in suids and for the construction and annotation of the genome sequence for the sex chromosomes. Our finding that the SSCYq repetitive content has corresponding sequence on the X chromosome gives further insight into structure of SSCY, and suggests further functionally important sequences remain to be discovered on the X and Y.
登录
查看更多内容
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
2.3
作者：
Raudsepp, T.;Das, P. J.;Chowdhary, B. P.
通讯作者：
Chowdhary, B. P.
影响因子：
14.8
作者：
Fiegler, Heike;Redon, Richard;Carter, Nigel P.
通讯作者：
Carter, Nigel P.
DOI：
10.1002/ajmg.b.30287
发表时间：
2006-04-05
影响因子：
2.8
作者：
Blasi, F;Bacchelli, E;Maestrini, E
通讯作者：
Maestrini, E
DOI：
10.1159/000134687
发表时间：
1997-01-01
期刊：
CYTOGENETICS AND CELL GENETICS
影响因子：
--
作者：
Rogel-Gaillard, C;Hayes, H;Vaiman, M
通讯作者：
Vaiman, M