Effects of the anti-gastric secretory drugs IT-066 and omeprazole mitogenic activities in the gastric juice of the rat.
Effects of the anti-gastric secretory drugs IT-066 and omeprazole mitogenic activities in the gastric juice of the rat.
复制标题
抗胃分泌药物IT-066和奥美拉唑促有丝分裂活性对大鼠胃液的影响。
DOI：
10.3109/00365529609031973
复制
发表时间：
1996
影响因子：
1.9
通讯作者：
M. Itoh
中科院分区：
文献类型：
--
作者：
K. Kasugai;T. Joh;H. Kataoka;T. Kato;M. Itoh
背景
唾液表皮生长因子（EGF）在胃液中保留了其生物学功能，并可能发挥生理作用。然而，关于EGF以外的有丝分裂原的存在以及抗促分泌素对胃液中这些因子的宪法改变知之甚少。
方法
通过测量[3 H]-胸苷掺入来评价促有丝分裂活性，并通过使用特异性抗大鼠EGF抗体来确定EGF贡献。H2受体拮抗剂（IT-066）和质子泵抑制剂（奥美拉唑）被用来确定这些药物是否改变胃液中活性有丝分裂原的相对组成。
结果
正常胃液显著增加DNA合成。该活性被抗体抑制（87-88%）。两种药物都能剂量依赖性地增加EGF的浓度和活性，IT-066特异性地增加EGF的总量和活性。大约50%的这种活性被煮沸或抗体降低。
结论
正常大鼠胃液的促有丝分裂活性主要依赖于EGF，而抗分泌药物通过保护和包括管腔内的有丝分裂原而增强促有丝分裂活性。
BACKGROUND
Salivary epidermal growth factor (EGF) retains its biologic function in gastric juice and may play a physiologic role. Little is know, however, about the existence of mitogens other than EGF and the constitutional alterations of these factors in gastric juice by anti-secretagogues.
METHODS
The mitogenic activity was evaluated by measuring [3H]-thymidine incorporation, and the EGF contribution was determined by using a specific anti-rat EGF antibody. An H2-receptor antagonist (IT-066) and a proton pump inhibitor (omeprazole) were used to determine whether these drugs alter the relative composition of active mitogens in gastric juice.
RESULTS
Normal gastric juice significantly increased DNA synthesis. This activity was suppressed by antibody (87-88%). Both drugs increased EGF concentrations and activity dose-dependently IT-066 specifically increased total amount and activity of EGF. Approximately 50% of this activity was reduced by boiling or antibody.
CONCLUSION
The major mitogenic activity of normal rat gastric juice depends on EGF, and antisecretory drugs enhance the mitogenic activity by preserving and including intraluminal mitogens than EGF.
影响因子：
4.8
作者：
M. G. Humphreys-Beher;S. Macauley;N. Chegini;G. B. V. Setten;K. Purushotham;Carol M. Stewart;Timothy T. Wheeler;Gregory S. Schultz
通讯作者：
M. G. Humphreys-Beher;S. Macauley;N. Chegini;G. B. V. Setten;K. Purushotham;Carol M. Stewart;Timothy T. Wheeler;Gregory S. Schultz
大鼠胃肠道中的 EGF 含量：年龄和禁食/进食的影响。
DOI：
10.1152/ajpgi.1989.256.5.g856
发表时间：
1989
期刊：
The American journal of physiology
影响因子：
--
作者：
Schaudies,RP;Grimes,J;Davis,D;Rao,RK;Koldovsky,O
通讯作者：
Koldovsky,O
早产儿胃液对表皮生长因子的最小水解。
影响因子：
24.5
作者：
Britton,JR;George-Nascimento,C;Udall,JN;Koldovský,O
通讯作者：
Koldovský,O