The mitochondrial genome of the land snail Camaenacicatricosa (Müller, 1774) (Stylommatophora, Camaenidae): the first complete sequence in the family Camaenidae.

The mitochondrial genome of the land snail Camaenacicatricosa (Müller, 1774) (Stylommatophora, Camaenidae): the first complete sequence in the family Camaenidae.
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陆地蜗牛 Camaena cicatricosa (Müller, 1774)(Stylommatophora,Camaenidae)的线粒体基因组:Camaenidae 科中第一个完整序列

DOI:
10.3897/zookeys.451.8537
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发表时间:
2014
期刊:
影响因子:
1.3
通讯作者:
Qian ZX
Qian ZX
中科院分区:
生物学3区
文献类型:
--
作者:
Wang P;Yang HF;Zhou WC;Hwang CC;Zhang WH;Qian ZX

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本研究测定了钉螺Camaena cicatricosa(Müler,1774)的线粒体全基因组。整个环状基因组全长13,843个碱基,代表了第一个双峰型mt基因组,其含量分别为31.9%A、37.9%T、13.5%C和16.7%G。基因含量、密码子使用和碱基组成与已测得的柱盖亚目mt基因组有很大的相似性,但基因顺序与之不同,特别是tRNACys、tRNAPhe、COII、tRNAAsp、tRNAGly、tRNAHis和tRNATrp的位置。除了COII以GTG为起始信号外,所有的蛋白质编码基因都使用标准起始密码子ATN。传统的终止密码子TAA和TAG已被分配给所有的蛋白质编码基因。所有的tRNA基因都具有典型的三叶草叶结构,但tRNAAsp的TψC臂和tRNAASer的二氢尿苷臂(Agn)只形成一个简单的环。在这组mt基因组中发现了较短的基因间隔区。基于蛋白质编码基因的系统发育研究表明,驼科和缓虫科的亲缘关系很近。所提出的系统发育与花柱亚纲的单系一致。
The complete mitochondrial (mt) genome of the snail Camaena cicatricosa (Müller, 1774) has been sequenced and annotated in this study. The entire circular genome is 13,843 bp in size and represents the first camaenid mt genome, with content of 31.9%A, 37.9%T, 13.5%C and 16.7%G. Gene content, codon usage and base organization show similarity to a great extent to the sequenced mt genome from Stylommatophora, whereas, gene order is different from them, especially the positions of tRNACys, tRNAPhe, COII, tRNAAsp, tRNAGly, tRNAHis and tRNATrp. All protein coding genes use standard initiation codons ATN except for COII with GTG as start signal. Conventional stop codons TAA and TAG have been assigned to all protein coding genes. All tRNA genes possess the typical clover leaf structure, but the TψC arm of tRNAAsp and dihydrouridine arm of tRNASer(AGN) only form a simple loop. Shorter intergenic spacers have been found in this mt genome. Phylogenetic study based on protein coding genes shows close relationship of Camaenidae and Bradybaenidae. The presented phylogeny is consistent with the monophyly of Stylommatophora.
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