The complete mitochondrial genome of Galba pervia (Gastropoda: Mollusca), an intermediate host snail of Fasciola spp.
The complete mitochondrial genome of Galba pervia (Gastropoda: Mollusca), an intermediate host snail of Fasciola spp.
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DOI：
10.1371/journal.pone.0042172
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Zhu XQ
中科院分区：
文献类型：
--
作者：
Liu GH;Wang SY;Huang WY;Zhao GH;Wei SJ;Song HQ;Xu MJ;Lin RQ;Zhou DH;Zhu XQ
全线粒体基因组和基因重排越来越多地被用作研究系统发育关系的分子标记。为完成腹足类，特别是肺螺类的mt基因组，我们确定了淡水蜗牛Galba pervia的mt基因组，Galba pervia是片形吸虫的重要中间宿主。在中国对G. pervia全长13，768 bp。其基因组呈环状，由37个基因组成，其中蛋白质基因13个，rRNA基因2个，tRNA基因22个。mt基因顺序为G. pervia的mt基因组序列与已测序的肺螺目物种的mt基因组序列相比显示了新的排列方式（tRNA-His、tRNA-Gly和tRNA-Tyr改变了位置和方向），表明肺螺目物种之间存在差异。共推导出3655个氨基酸，编码13个蛋白质基因。使用频率最高的氨基酸是Leu（15.05%），其次是Phe（11.24%）、Ser（10.76%）和IIe（8.346%）。系统发育分析使用串联的13个蛋白质编码基因的氨基酸序列，与三种不同的计算算法（最大简约，最大似然和贝叶斯分析），都显示，家庭的螺和Planorbidae密切相关的两个蜗牛家庭，与以前的分类基于形态和分子研究。对G. Pervia的基因排列是一种新的基因排列方式，它代表了第一个测序的高质量的松鼠科mt基因组。这些新的mtDNA数据为研究淡水螺的流行病学、群体遗传学和种群地理学提供了额外的遗传标记，也为了解中间螺宿主和片形吸虫属内软体动物阶段之间的相互作用提供了额外的遗传标记。
Complete mitochondrial (mt) genomes and the gene rearrangements are increasingly used as molecular markers for investigating phylogenetic relationships. Contributing to the complete mt genomes of Gastropoda, especially Pulmonata, we determined the mt genome of the freshwater snail Galba pervia, which is an important intermediate host for Fasciola spp. in China. The complete mt genome of G. pervia is 13,768 bp in length. Its genome is circular, and consists of 37 genes, including 13 genes for proteins, 2 genes for rRNA, 22 genes for tRNA. The mt gene order of G. pervia showed novel arrangement (tRNA-His, tRNA-Gly and tRNA-Tyr change positions and directions) when compared with mt genomes of Pulmonata species sequenced to date, indicating divergence among different species within the Pulmonata. A total of 3655 amino acids were deduced to encode 13 protein genes. The most frequently used amino acid is Leu (15.05%), followed by Phe (11.24%), Ser (10.76%) and IIe (8.346%). Phylogenetic analyses using the concatenated amino acid sequences of the 13 protein-coding genes, with three different computational algorithms (maximum parsimony, maximum likelihood and Bayesian analysis), all revealed that the families Lymnaeidae and Planorbidae are closely related two snail families, consistent with previous classifications based on morphological and molecular studies. The complete mt genome sequence of G. pervia showed a novel gene arrangement and it represents the first sequenced high quality mt genome of the family Lymnaeidae. These novel mtDNA data provide additional genetic markers for studying the epidemiology, population genetics and phylogeographics of freshwater snails, as well as for understanding interplay between the intermediate snail hosts and the intra-mollusca stages of Fasciola spp..
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影响因子：
4.1
作者：
Knudsen, B;Kohn, AB;Moroz, LL
通讯作者：
Moroz, LL
影响因子：
6.5
作者：
Hassanin, A;Léger, N;Deutsch, J
通讯作者：
Deutsch, J
影响因子：
2
作者：
Cai, X. Q.;Liu, G. H.;Zhu, X. Q.
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影响因子：
64.8
作者：
Kocot, Kevin M.;Cannon, Johanna T.;Todt, Christiane;Citarella, Mathew R.;Kohn, Andrea B.;Meyer, Achim;Santos, Scott R.;Schander, Christoffer;Moroz, Leonid L.;Lieb, Bernhard;Halanych, Kenneth M.
通讯作者：
Halanych, Kenneth M.
腹足动物线粒体基因组排列的进化。
影响因子：
3.4
作者：
Grande, Cristina;Templado, Jose;Zardoya, Rafael
通讯作者：
Zardoya, Rafael