Disentangling pectic homogalacturonan and rhamnogalacturonan-I polysaccharides: Evidence for sub-populations in fruit parenchyma systems.
Disentangling pectic homogalacturonan and rhamnogalacturonan-I polysaccharides: Evidence for sub-populations in fruit parenchyma systems.
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DOI：
10.1016/j.foodchem.2017.11.025
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发表时间：
2018-04-25
期刊：
影响因子：
8.8
通讯作者：
Knox JP
中科院分区：
文献类型：
--
作者：
Cornuault V;Posé S;Knox JP
四种水果薄壁系统细胞壁多糖的解剖。HG分子具有与甲基酯化HG分离的未酯化区域。RG-I结构域存在于HG相关和非HG相关两种形式。在所有水果中检测到可溶性木葡聚糖和果胶相关木葡聚糖。茄子薄壁组织中果胶-木葡聚糖-木聚糖复合物的检测。植物细胞壁基质多糖是影响细胞壁、组织和器官性质的多种可变聚合物。以相对简单的四种水果（番茄、茄子、草莓和苹果）的薄壁组织为研究对象，我们利用顺序增溶和基于抗体的方法分析了细胞壁基质多糖的含量，重点研究了果胶均半乳糖醛酸（HG）和鼠李糖半乳糖醛酸- i （RG-I）。结合阴离子交换色谱分析的表位检测表明，在所有情况下，溶解的聚合物包括具有未酯化区域的HG分子光谱，这些区域与甲基化的HG结构域是可分离的。在高可溶性馏分中，RG-I结构域以hg相关和非hg相关的形式存在。在所有果实中均检测到可溶性木葡聚糖和果胶相关木葡聚糖成分。茄子聚糖含有丰富的杂氧聚糖表位，其中一些表位与果胶和木葡聚糖都有关。这些多糖异质性的概况为未来更复杂的细胞和组织系统的研究提供了基础。
Dissection of cell wall polysaccharides from four fruit parenchyma systems. HG molecules with unesterified regions separable from methyl esterified HG. RG-I domains exist in both HG-associated and non-HG-associated forms. Soluble xyloglucan and pectin-associated xyloglucan detected in all fruits. Detection of pectin-xyloglucan-xylan complexes in aubergine parenchyma. The matrix polysaccharides of plant cell walls are diverse and variable sets of polymers influencing cell wall, tissue and organ properties. Focusing on the relatively simple parenchyma tissues of four fruits – tomato, aubergine, strawberry and apple – we have dissected cell wall matrix polysaccharide contents using sequential solubilisation and antibody-based approaches with a focus on pectic homogalacturonan (HG) and rhamnogalacturonan-I (RG-I). Epitope detection in association with anion-exchange chromatography analysis indicates that in all cases solubilized polymers include spectra of HG molecules with unesterified regions that are separable from methylesterified HG domains. In highly soluble fractions, RG-I domains exist in both HG-associated and non-HG-associated forms. Soluble xyloglucan and pectin-associated xyloglucan components were detected in all fruits. Aubergine glycans contain abundant heteroxylan epitopes, some of which are associated with both pectin and xyloglucan. These profiles of polysaccharide heterogeneity provide a basis for future studies of more complex cell and tissue systems.
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影响因子：
3.1
作者：
Hoffman, M;Jia, ZH;York, WS
通讯作者：
York, WS
影响因子：
8.8
作者：
ESTEBAN, RM;LOPEZANDREU, FJ;MOLLA, E
通讯作者：
MOLLA, E
果皮同质分离剂掩盖了植物细胞壁中丰富的木葡聚糖表位。
影响因子：
5.3
作者：
Marcus SE;Verhertbruggen Y;Hervé C;Ordaz-Ortiz JJ;Farkas V;Pedersen HL;Willats WG;Knox JP
通讯作者：
Knox JP
影响因子：
4.8
作者：
Pedersen, Henriette L.;Fangel, Jonatan U.;Willats, William G. T.
通讯作者：
Willats, William G. T.
影响因子：
10.7
作者：
Lin, Dehui;Lopez-Sanchez, Patricia;Gidley, Michael J.
通讯作者：
Gidley, Michael J.