TCAB1: a potential target for diagnosis and therapy of head and neck carcinomas.
TCAB1: a potential target for diagnosis and therapy of head and neck carcinomas.
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TCAB1：头颈癌诊断和治疗的潜在靶点
DOI：
10.1186/1476-4598-13-180
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发表时间：
2014-07-28
期刊：
影响因子：
37.3
通讯作者：
Chen QM
中科院分区：
文献类型：
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作者：
Sun CK;Luo XB;Gou YP;Hu L;Wang K;Li C;Xiang ZT;Zhang P;Kong XL;Zhang CL;Yang Q;Li J;Xiao LY;Li Y;Chen QM
WRAP 53（包括α、β和γ亚型）不仅在p53 mRNA的稳定性中起重要作用，而且在端粒酶全酶的组装和运输中起重要作用。它被认为是一种致癌基因，被认为可以促进癌细胞的存活。本研究旨在探讨TCAB 1（WRAP 53 α除外）在头颈部肿瘤发生发展中的作用。采用免疫组化法检测TCAB 1在临床标本切片中的表达，并采用蛋白质印迹法检测TCAB 1在细胞系中的表达水平。使用shRNA慢病毒耗尽TCAB 1，并使用q-PCR和Western印迹评估敲低效率。采用CCK-8法和流式细胞仪检测细胞增殖能力，并采用transwell法检测细胞体外侵袭能力。异种移植物用于检测体内成瘤潜力。此外，我们使用cDNA微阵列来研究参与这一过程的候选因子。我们观察到TCAB 1在头颈癌临床标本以及癌细胞系中显著过表达。TCAB 1基因的敲除可降低细胞的体外增殖能力和侵袭能力。cDNA微阵列分析表明，TCAB 1介导的癌症调控可能涉及与肿瘤发生和癌症发展相关的几种途径和因子。此外，异种移植物测定证实TCAB 1的耗尽将抑制裸鼠中的肿瘤形成。免疫组化结果显示，shTCAB 1移植瘤细胞增殖能力下降，凋亡趋势增强，肿瘤血管生成受到抑制。我们的研究表明，TCAB 1的耗尽降低细胞增殖和侵袭潜力在体外和体内。提示TCAB 1可能促进头颈部肿瘤的发生和发展。TCAB 1有可能作为头颈部肿瘤的预后标志物或潜在的诊断和治疗靶点。
WRAP53, including α, β and γ isoforms, plays an important role not only in the stability of p53 mRNA, but also in the assembly and trafficking of the telomerase holoenzyme. It has been considered an oncogene and is thought to promote the survival of cancer cells. The aim of this study was to detect the role of TCAB1 (except WRAP53α) in the occurrence and development of head and neck carcinomas. Immunohistochemistry was used to detect the TCAB1 expression in clinical specimen sections and performed western blotting to check the TCAB1 expression levels in cell lines. TCAB1 was depleted using shRNA lentivirus and the knockdown efficiency was assessed using q-PCR and Western blotting. We performed CCK-8 assays and flow cytometry to check the cell proliferation potential and used the trans-well assay to test the invasion ability in vitro. Xenografts were used to detect the tumor formation potential in vivo. Moreover, we performed cDNA microarray to investigate the candidate factors involved in this process. We observed a notable overexpression of TCAB1 in head and neck carcinoma clinical specimens as well as in carcinoma cell lines. Knockdown of TCAB1 decreased the cellular proliferation potential and invasion ability in vitro. cDNA microarray analysis suggested the possible involvement of several pathways and factors associated with tumorigenesis and carcinoma development in the TCAB1-mediated regulation of cancers. Furthermore, the xenograft assay confirmed that the depletion of TCAB1 would inhibit tumor formation in nude mice. The immunohistochemistry results of the mice tumor tissue sections revealed that the cells in shTCAB1 xenografts showed decreased proliferation potential and increased apoptotic trend, meanwhile, the angiogenesis was inhibited in the smaller tumors form shTCAB1 cells. Our study demonstrated that depletion of TCAB1 decreased cellular proliferation and invasion potential both in vitro and in vivo. The data indicated that TCAB1 might facilitate the occurrence and development of head and neck carcinomas. In future, TCAB1 might be useful as a prognostic biomarker or a potential target for the diagnosis and therapy of head and neck carcinomas.
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影响因子：
14.9
作者：
Wu, Jyun-Yi;Chen, Chia-Hsin;Yeh, Li-Yin;Yeh, Ming-Long;Ting, Chun-Chan;Wang, Yan-Hsiung
通讯作者：
Wang, Yan-Hsiung
影响因子：
9.8
作者：
Mahmoudi S;Henriksson S;Weibrecht I;Smith S;Söderberg O;Strömblad S;Wiman KG;Farnebo M
通讯作者：
Farnebo M
影响因子：
5.3
作者：
Stern, J. Lewis;Zyner, Katherine G.;Bryan, Tracy M.
通讯作者：
Bryan, Tracy M.
影响因子：
2.9
作者：
Upreti D;Pathak A;Kung SK
通讯作者：
Kung SK
影响因子：
9
作者：
通讯作者：
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