Migration and division in cell monolayers on substrates with topological defects.
Migration and division in cell monolayers on substrates with topological defects.
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DOI：
10.1073/pnas.2301197120
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发表时间：
2023-07-25
影响因子：
11.1
通讯作者：
Camley, Brian A.
中科院分区：
文献类型：
--
作者：
Kaiyrbekov, Kurmanbek;Endresen, Kirsten;Sullivan, Kyle;Zheng, Zhaofei;Chen, Yun;Serra, Francesca;Camley, Brian A.
当细长的细胞以高密度存在时，例如在组织中，它们的长轴倾向于彼此对齐。这种顺序并不完美，它失败的地方-缺陷-可以用来控制组织的属性，引导细胞死亡和组织运动。在这里，我们将细长的成纤维细胞放在诱导缺陷的脊状图案上。这些缺陷改变细胞密度，但不是通过改变细胞爬行运动，如在其他细胞类型中发现的那样。相反，我们认为，成纤维细胞分裂是敏感的细胞形状和细胞形状的改变，我们使用的模式。我们使用建模，这个过程可以解释我们的实验结果。我们的工作提供了一套额外的工具来控制和组织组织。细胞单层的集体运动和组织对于伤口愈合和组织发育是重要的。最近的实验强调了这些层中液晶顺序的重要性，表明+1拓扑缺陷在组织组织形态发生中起作用。我们研究了成纤维细胞的组织，运动和增殖的基板与微米大小的脊，诱导+1和-1拓扑缺陷，使用模拟和实验。我们将细胞建模为通过Gay-Berne势相互作用的自推进可变形椭圆。与其他细胞类型的早期工作不同，我们看到缺陷附近的密度变化不能用集体迁移来解释。相反，我们建议成纤维细胞根据其面积和纵横比具有不同的分裂速率。该模型捕捉了我们之前实验的关键特征：在高细胞密度下的对准质量是稳定的，并且在+1缺陷的中心，细胞可以采用高度各向异性或主要各向同性的形态。用不同脊高度进行的实验证实了该模型的预测：抑制跨脊的迁移促进+1缺陷处的更高细胞密度。我们的工作使得能够使用拓扑缺陷而不依赖于细胞迁移的组织图案化机制。
When elongated cells exist at high densities, such as in tissues, their long axes tend to align with each other. This order is not perfect, and places where it fails—defects—can be used to control the tissue’s properties, guiding cell death and tissue motion. Here, we place elongated fibroblasts on ridged patterns that induce defects. These defects change cell density—but not by changing cell crawling motion, as found in other cell types. We instead argue that fibroblast division is sensitive to cell shape and cell shape is changed by the pattern we use. We show using modeling that this process can explain our experimental results. Our work provides an additional set of tools to control and organize tissues. Collective movement and organization of cell monolayers are important for wound healing and tissue development. Recent experiments highlighted the importance of liquid crystal order within these layers, suggesting that +1 topological defects have a role in organizing tissue morphogenesis. We study fibroblast organization, motion, and proliferation on a substrate with micron-sized ridges that induce +1 and −1 topological defects using simulation and experiment. We model cells as self-propelled deformable ellipses that interact via a Gay–Berne potential. Unlike earlier work on other cell types, we see that density variation near defects is not explained by collective migration. We propose instead that fibroblasts have different division rates depending on their area and aspect ratio. This model captures key features of our previous experiments: the alignment quality worsens at high cell density and, at the center of the +1 defects, cells can adopt either highly anisotropic or primarily isotropic morphologies. Experiments performed with different ridge heights confirm a prediction of this model: Suppressing migration across ridges promotes higher cell density at the +1 defect. Our work enables a mechanism for tissue patterning using topological defects without relying on cell migration.
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DOI：
10.1103/physreve.89.062705
发表时间：
2014-06
期刊：
Physical review. E, Statistical, nonlinear, and soft matter physics
影响因子：
--
作者：
Camley BA;Rappel WJ
通讯作者：
Rappel WJ
DOI：
10.1103/physreve.95.012401
发表时间：
2017-01
期刊：
Physical review. E
影响因子：
--
作者：
Camley BA;Zhao Y;Li B;Levine H;Rappel WJ
通讯作者：
Rappel WJ
影响因子：
16.6
作者：
Großmann R;Aranson IS;Peruani F
通讯作者：
Peruani F
DOI：
10.1073/pnas.1219937110
发表时间：
2013-02-12
影响因子：
11.1
作者：
Basan, Markus;Elgeti, Jens;Levine, Herbert
通讯作者：
Levine, Herbert
影响因子：
3.1
作者：
Calderon-Alcaraz, A.;Munguia-Valadez, J.;Moreno-Razo, J. A.
通讯作者：
Moreno-Razo, J. A.