Acoustic overexposure increases the expression of VGLUT-2 mediated projections from the lateral vestibular nucleus to the dorsal cochlear nucleus.
Acoustic overexposure increases the expression of VGLUT-2 mediated projections from the lateral vestibular nucleus to the dorsal cochlear nucleus.
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DOI：
10.1371/journal.pone.0035955
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Hamann M
中科院分区：
文献类型：
--
作者：
Barker M;Solinski HJ;Hashimoto H;Tagoe T;Pilati N;Hamann M
耳蜗背核（DCN）是中枢听觉系统的第一中继，也是多模态信息整合的场所。囊泡谷氨酸转运蛋白VGLUT-1和VGLUT-2选择性地将谷氨酸包装到突触囊泡中，并且发现在DCN中具有不同的组织模式。听觉神经纤维主要与VGLUT-1共标记，而体感输入主要与VGLUT-2共标记。在这里，我们使用逆行和顺行运输的荧光共轭葡聚糖胺（DA），以证明前庭外侧核（LVN）表现出同侧的投射到梭形和深层的大鼠DCN。刺激LVN可在梭形细胞和颗粒细胞中间神经元上诱发突触能电流。我们结合葡聚糖胺神经元示踪方法与免疫组织化学，并表明标记的预测LVN的共同标记与VGLUT-2的VGLUT-1相比。Wistar大鼠暴露于高强度的单音（15 kHz，110 dB SPL）6小时。声学过度暴露后5天，DCN中VGLUT-1的表达水平降低，而DCN中VGLUT-2的表达水平增加，包括起源于LVN的终末。VGLUT-2介导的从LVN到DCN的投射可能在响应声音的头部位置中起作用。听觉过度刺激后VGLUT-2表达的增强可能是前庭输入对听力损失和听神经纤维VGLUT-1表达减少的一种代偿机制。
The dorsal cochlear nucleus (DCN) is a first relay of the central auditory system as well as a site for integration of multimodal information. Vesicular glutamate transporters VGLUT-1 and VGLUT-2 selectively package glutamate into synaptic vesicles and are found to have different patterns of organization in the DCN. Whereas auditory nerve fibers predominantly co-label with VGLUT-1, somatosensory inputs predominantly co-label with VGLUT-2. Here, we used retrograde and anterograde transport of fluorescent conjugated dextran amine (DA) to demonstrate that the lateral vestibular nucleus (LVN) exhibits ipsilateral projections to both fusiform and deep layers of the rat DCN. Stimulating the LVN induced glutamatergic synaptic currents in fusiform cells and granule cell interneurones. We combined the dextran amine neuronal tracing method with immunohistochemistry and showed that labeled projections from the LVN are co-labeled with VGLUT-2 by contrast to VGLUT-1. Wistar rats were exposed to a loud single tone (15 kHz, 110 dB SPL) for 6 hours. Five days after acoustic overexposure, the level of expression of VGLUT-1 in the DCN was decreased whereas the level of expression of VGLUT-2 in the DCN was increased including terminals originating from the LVN. VGLUT-2 mediated projections from the LVN to the DCN are likely to play a role in the head position in response to sound. Amplification of VGLUT-2 expression after acoustic overexposure could be a compensatory mechanism from vestibular inputs in response to hearing loss and to a decrease of VGLUT-1 expression from auditory nerve fibers.
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