A comparison of methods to assess the antimicrobial activity of nanoparticle combinations on bacterial cells.
A comparison of methods to assess the antimicrobial activity of nanoparticle combinations on bacterial cells.
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DOI：
10.1371/journal.pone.0192093
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Ciric L
中科院分区：
文献类型：
--
作者：
Bankier C;Cheong Y;Mahalingam S;Edirisinghe M;Ren G;Cloutman-Green E;Ciric L
暴露于抗菌化合物后的细菌细胞定量在整个行业和医疗保健中变化很大。许多方法被用来量化这些抗菌效果。随着对疾病控制新预防方法的需求不断增加，我们旨在比较和评估用于确定新型纳米颗粒组合对两种不同病原体的抗菌作用的常用分析方法。使用总活细胞的平板计数、流式细胞术（LIVE/DEAD BacLight活力测定）和qPCR（活力qPCR）来评估不同浓度（0.05、0.10和0.25 w/v%）的工程化纳米颗粒组合（NPC）对革兰氏阳性（金黄色葡萄球菌）和革兰氏阴性（铜绿假单胞菌）细菌的抗微生物活性。使用线性模型分析结果，以评估不同治疗的有效性。三种NPC（AMNP 0 -2）对两种病原体的强烈抗菌作用可以使用平板计数法和流式细胞术进行量化。平板计数法显示，暴露于高NPC浓度的细菌的对数减少量很高（>8-log）。我们使用流式细胞术活/死测定法发现了类似的抗菌结果。由于NPC干扰qPCR扩增，因此无法定量抗微生物活性的活力qPCR分析。流式细胞术被确定为测量新型NPC的抗微生物活性的最佳方法，这是由于高通量、快速和可量化的结果。
Bacterial cell quantification after exposure to antimicrobial compounds varies widely throughout industry and healthcare. Numerous methods are employed to quantify these antimicrobial effects. With increasing demand for new preventative methods for disease control, we aimed to compare and assess common analytical methods used to determine antimicrobial effects of novel nanoparticle combinations on two different pathogens. Plate counts of total viable cells, flow cytometry (LIVE/DEAD BacLight viability assay) and qPCR (viability qPCR) were used to assess the antimicrobial activity of engineered nanoparticle combinations (NPCs) on Gram-positive (Staphylococcus aureus) and Gram-negative (Pseudomonas aeruginosa) bacteria at different concentrations (0.05, 0.10 and 0.25 w/v%). Results were analysed using linear models to assess the effectiveness of different treatments. Strong antimicrobial effects of the three NPCs (AMNP0–2) on both pathogens could be quantified using the plate count method and flow cytometry. The plate count method showed a high log reduction (>8-log) for bacteria exposed to high NPC concentrations. We found similar antimicrobial results using the flow cytometry live/dead assay. Viability qPCR analysis of antimicrobial activity could not be quantified due to interference of NPCs with qPCR amplification. Flow cytometry was determined to be the best method to measure antimicrobial activity of the novel NPCs due to high-throughput, rapid and quantifiable results.
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影响因子：
4.2
作者：
Pan H;Zhang Y;He GX;Katagori N;Chen H
通讯作者：
Chen H
影响因子：
4.8
作者：
Nestorovich, Ekaterina M.;Sugawara, Etsuko;Bezrukov, Sergey M.
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作者：
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通讯作者：
Obst, Ursula
DOI：
10.1016/j.nano.2006.12.001
发表时间：
2007-03-01
影响因子：
5.4
作者：
Kim, Jun Sung;Kuk, Eunye;Cho, Myung-Haing
通讯作者：
Cho, Myung-Haing