Antibody-based detection and inhibition of vaginolysin, the Gardnerella vaginalis cytolysin.
Antibody-based detection and inhibition of vaginolysin, the Gardnerella vaginalis cytolysin.
复制标题
DOI：
10.1371/journal.pone.0005207
复制
发表时间：
2009
期刊：
影响因子：
3.7
通讯作者：
Ratner AJ
中科院分区：
文献类型：
--
作者：
Randis TM;Kulkarni R;Aguilar JL;Ratner AJ
Bacterial vaginosis (BV) is the most common vaginal infection worldwide and is associated with significant adverse sequelae. We have recently characterized vaginolysin (VLY), the human-specific cytotoxin produced by Gardnerella vaginalis and believed to play a critical role in the pathogenesis of BV and its associated morbidities. We hypothesize that novel antibody-based strategies may be useful for detection of VLY and for inhibition of its toxic effects on human cells. Using purified toxin as an immunogen, we generated polyclonal rabbit immune serum (IS) against VLY. A western blot of G. vaginalis lysate was probed with IS and a single band (57 kD) identified. Immunofluorescence techniques using IS detected VLY production by G. vaginalis. In addition, we have developed a sandwich ELISA assay capable of VLY quantification at ng/ml concentrations in the supernatant of growing G. vaginalis. To investigate the potential inhibitory role of IS on VLY-mediated cell lysis, we exposed human erythrocytes to VLY or VLY pretreated with IS and determined the percent hemolysis. Pretreatment with IS resulted in a significant reduction in VLY-mediated lysis. Similarly, both human cervical carcinoma cells and vaginal epithelial cells exhibited reduced cytolysis following exposure to VLY with IS compared to VLY alone. These results confirm that antibody-based techniques are an effective means of VLY detection. Furthermore, VLY antiserum functions as an inhibitor of VLY–CD59 interaction, mitigating cell lysis. These strategies may have a potential role in the diagnosis and treatment of BV.
登录
查看更多内容
影响因子：
9.4
作者：
BRISELDEN, AM;HILLIER, SL
通讯作者：
HILLIER, SL
DOI：
10.1155/s1064744997000380
发表时间：
1997-01-01
影响因子：
--
作者：
Calderon, E;Rivera, R;Conde-Glez, C
通讯作者：
Conde-Glez, C
影响因子：
6.4
作者：
Hedges, SR;Barrientes, F;Schwebke, JR
通讯作者：
Schwebke, JR
影响因子：
158.5
作者：
Hillier, SL;Nugent, RP;Klebanoff, MA
通讯作者：
Klebanoff, MA
影响因子：
6.4
作者：
Martin, HL;Richardson, BA;Kreiss, J
通讯作者：
Kreiss, J