Germ cell binding to rat Sertoli cells in vitro.
Germ cell binding to rat Sertoli cells in vitro.
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生殖细胞在体外与大鼠支持细胞结合。
DOI：
10.1095/biolreprod37.5.1271
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发表时间：
1987
影响因子：
3.6
通讯作者：
Danahey,DG
中科院分区：
文献类型：
--
作者：
DePhilip,RM;Danahey,DG
通过使用分离的大鼠生殖细胞和支持细胞的原代培养物的共孵育实验，在体外研究了雄性生殖细胞和支持细胞之间的相互作用，通过生殖细胞对低渗休克的不同敏感性使支持细胞不含生殖细胞。使用相差和扫描电子显微镜对生殖细胞/支持细胞的相互作用进行形态学检查，然后通过测量与添加的[3H]亮氨酸标记的生殖细胞共孵育后与支持细胞培养物结合的放射性来定量。生殖细胞与支持细胞培养物的结合是这两种细胞类型之间特异性粘附的结果，并且观察到了这种特异性粘附的几个特征。首先，生殖细胞在脾细胞和红细胞不粘附的条件下粘附到支持细胞培养物上。其次，生殖细胞对支持细胞培养物的亲和力比对睾丸管周细胞或小脑星形胶质细胞培养物的亲和力更大。第三，用多聚甲醛固定的生殖细胞粘附到活的支持培养物上，而类似固定的脾细胞粘附不太紧密。活的生殖细胞和多聚甲醛固定的生殖细胞都不会粘附到固定的支持细胞培养物上。第四，生殖细胞与支持细胞培养物的结合不是立即的，而是稳定增加，并在共孵育 4 小时时达到最大值。当每平方毫米支持细胞培养物表面添加超过 4200 个生殖细胞时，生殖细胞与支持细胞培养物的结合就会饱和。最后，当在冰上进行共孵育时，生殖细胞与支持细胞培养物的结合被消除。基于这些观察，我们得出结论，生殖细胞对支持细胞的粘附是特异性的、温度依赖性的，并且需要可行的支持细胞，但不一定是可行的生殖细胞。这些结果对于理解生精小管内支持细胞和生殖细胞之间复杂的相互作用以及设计未来探索这种相互作用细节的实验具有重要意义。
The interaction between male germ cells and Sertoli cells was studied in vitro by co-incubation experiments using isolated rat germ cells and primary cultures of Sertoli cells made germ cell-free by the differential sensitivity of germ cells to hypotonic shock. The germ cell/Sertoli cell interaction was examined morphologically with phase-contrast and scanning electron microscopy and then quantified by measuring radioactivity bound to Sertoli cell cultures after co-incubation with added [3H] leucine-labeled germ cells. Germ cell binding to Sertoli cell cultures was the result of specific adhesion between these two cell types, and several features of this specific adhesion were observed. First, germ cells adhered to Sertoli cell cultures under conditions during which spleen cells and red blood cells did not. Second, germ cells had a greater affinity for Sertoli cell cultures than they had for cultures of testicular peritubular cells or cerebellar astrocytes. Third, germ cells fixed with paraformaldehyde adhered to live Sertoli cultures while similarly fixed spleen cells adhered less tightly. Neither live nor paraformaldehyde-fixed germ cells adhered to fixed Sertoli cell cultures. Fourth, germ cell binding to Sertoli cell cultures was not immediate but increased steadily and approached a maximum at 4 h of co-incubation. Saturation of germ cell binding to Sertoli cell cultures occurred when more than 4200 germ cells were added per mm2 of Sertoli cell culture surface. Finally, germ cell binding to Sertoli cell cultures was eliminated when co-incubation was performed on ice. Based on these observations, we concluded that germ cell adhesion to Sertoli cells was specific, temperature-dependent, and required a viable Sertoli cell but not necessarily a viable germ cell. These results have important implications for understanding the complex interaction between Sertoli cells and germ cells within the seminiferous tubule and in the design of future experiments probing details of this interaction.
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大鼠支持细胞和生殖细胞之间相互作用的证据
DOI：
--
发表时间：
1980
期刊：
影响因子：
--
作者：
M. Stefanini;E. Ziparo;F. Palombi;M. Galdieri;R. Geremia;M. Russo
通讯作者：
M. Russo
生殖细胞刺激支持细胞蛋白磷酸化。
影响因子：
4.8
作者：
Ireland,ME;Welsh,MJ
通讯作者：
Welsh,MJ
影响因子：
4.8
作者：
HUTSON, JC;STOCCO, DM
通讯作者：
STOCCO, DM
支持细胞分泌雄激素结合蛋白受到与生殖细胞接触的影响。
影响因子：
--
作者：
M. Galdieri;L. Monaco;M. Stefanini
通讯作者：
M. Stefanini
伴刀豆球蛋白 A 诱导生精细胞在体外附着于支持细胞。
影响因子：
3.7
作者：
J. Grootegoed;N. Jutte;F. Rommerts;H. van der Molen;S. Ohno
通讯作者：
S. Ohno