From EST to novel spider silk gene identification for production of spidroin-based biomaterials.
From EST to novel spider silk gene identification for production of spidroin-based biomaterials.
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从 EST 到新型蜘蛛丝基因鉴定，用于生产基于蜘蛛蛋白的生物材料
DOI：
10.1038/s41598-017-13876-1
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发表时间：
2017-10-17
影响因子：
4.6
通讯作者：
Lin Z
中科院分区：
文献类型：
--
作者：
Huang W;Zhang Y;Chen Y;Wang Y;Yuan W;Zhang N;Lam TJ;Gong Z;Yang D;Lin Z
从一个热带蜘蛛种的全部7个丝腺池中构建了cDNA文库。共建立了1000多个表达序列标签（EST）克隆。大约65%的EST克隆被鉴定，大约50%被注释。EST克隆的细胞和功能分布表明其在蜘蛛丝腺中具有较高的蛋白质合成活性。蜘蛛丝基因的一个重要特征是氨基酸序列的重复。其中一个克隆，即目前研究中的TuSp2，包含两个几乎相同的片段，具有一个短的c端结构域。反转录（RT） PCR和表达分析表明，该基因在小管状腺中表达，参与卵丝的形成。此外，它的单一重复区域可以被诱导形成各种类型的材料，包括宏观纤维、透明薄膜和半透明水凝胶。该研究为未来鉴定新型蜘蛛素和开发新型蜘蛛素生物材料提供了广阔的前景。
A cDNA library from a pool of all the seven silk glands from a tropical spider species was constructed. More than 1000 expressed sequence tag (EST) clones were created. Almost 65% of the EST clones were identified and around 50% were annotated. The cellular and functional distribution of the EST clones indicated high protein synthesis activity in spider silk glands. Novel clones with repetitive amino acid sequences, which is one of the most important characteristics of spider silk genes, were isolated. One of these clones, namely TuSp2 in current research, contains two almost identical fragments with one short C-terminal domain. Reverse transcription (RT) PCR and expression analysis showed that it is expressed in the tubuliform gland and involved in eggcase silk formation. Furthermore, its single repetitive domain can be induced to form various types of materials, including macroscopic fibers, transparent film and translucent hydrogel. This study implies promising potentials for future identification of novel spidroins and development of new spidroin-based biomaterials.
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影响因子：
56.9
作者：
Guerette, PA;Ginzinger, DG;Gosline, JM
通讯作者：
Gosline, JM
影响因子：
4.8
作者：
Hu, XY;Kohler, K;Vierra, C
通讯作者：
Vierra, C
DOI：
10.1073/pnas.0813255106
发表时间：
2009-06-02
影响因子：
11.1
作者：
Lin, Zhi;Huang, Weidong;Yang, Daiwen
通讯作者：
Yang, Daiwen
影响因子：
5.6
作者：
Hayashi, CY;Lewis, RV
通讯作者：
Lewis, RV
影响因子：
3.8
作者：
Beckwitt, R;Arcidiacono, S;Stote, R
通讯作者：
Stote, R