A fixed-time diffusion analysis method determines that the three cheV genes of Helicobacter pylori differentially affect motility.
A fixed-time diffusion analysis method determines that the three cheV genes of Helicobacter pylori differentially affect motility.
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DOI：
10.1099/mic.0.021857-0
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发表时间：
2009-04
期刊：
影响因子：
--
通讯作者：
Ottemann KM
中科院分区：
文献类型：
--
作者：
Lowenthal AC;Simon C;Fair AS;Mehmood K;Terry K;Anastasia S;Ottemann KM
Helicobacter pylori is a chemotactic bacterium that has three CheV proteins in its predicted chemotaxis signal transduction system. CheV proteins contain both CheW- and response regulator-like domains. To determine the function of these proteins, we developed a fixed-time diffusion method that would quantify bacterial direction change without needing to define particular behaviors, to deal with the many behaviors that swimming H. pylori exhibit. We then analyzed mutants that had each cheV gene deleted individually and found that the behavior of each mutant differed substantially from wild type and each other. cheV1 and cheV2 mutants displayed smooth swimming behavior, consistent with decreased cellular CheY-P, similar to a cheW mutant. In contrast, the cheV3 mutant had the opposite effect and appeared to change direction frequently. Additional analysis showed that the cheV mutants displayed aberrant behavior as compared to the wild type in the soft agar chemotaxis assay. The soft agar assay phenotype was less extreme compared to that seen in the fixed-time diffusion model, suggesting that the cheV mutants are able to partially compensate for their defects under some conditions. Each cheV mutant furthermore had defects in mouse colonization that ranged from severe to modest, consistent with a role in chemotaxis. These studies thus show that the H. pylori CheV proteins each differently affect swimming behavior.
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