Gene expression profiling of intestinal regeneration in the sea cucumber.
Gene expression profiling of intestinal regeneration in the sea cucumber.
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DOI：
10.1186/1471-2164-10-262
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发表时间：
2009-06-08
期刊：
影响因子：
4.4
通讯作者：
García-Arrarás JE
中科院分区：
文献类型：
--
作者：
Ortiz-Pineda PA;Ramírez-Gómez F;Pérez-Ortiz J;González-Díaz S;Santiago-De Jesús F;Hernández-Pasos J;Del Valle-Avila C;Rojas-Cartagena C;Suárez-Castillo EC;Tossas K;Méndez-Merced AT;Roig-López JL;Ortiz-Zuazaga H;García-Arrarás JE
在后口动物中，棘皮动物的再生潜力最大，棘皮动物可以迅速取代大多数受损的器官。特别是，海参是研究器官再生的优秀模型，因为它们在剔除内脏后会再生消化道。然而，棘皮动物在现代再生研究中一直被边缘化，部分原因是缺乏现代基因组工具所提供的全基因组图谱方法。在过去的十年里，我们的实验室一直在使用海参来剖析细胞和分子事件，这些事件使得这种令人惊叹的再生过程成为可能。我们已经建立了一个EST数据库，该数据库来自正常和再生肠道在两个不同的再生阶段的cDNA文库。这个数据库现在有超过7000个序列。在目前的工作中，我们使用了安捷伦公司定制的带有60个EST探针的芯片，来确定肠道再生过程中的基因表达谱。在这里，我们比较了动物在三个不同的肠道再生阶段(摘除内脏后3天、7天和14天)的表达谱与正常(未剔除的)肠道的表达谱。差异表达的探针数量从P<；0.001的70%到P<；0.001的39%不等。聚类分析显示了早期(第一周)和后期(第二周)再生阶段的特定表达谱。我们用半定量逆转录聚合酶链式反应(RT-PCR)验证了芯片检测到的15个差异表达基因的表达谱，两种技术的符合率超过86%。大多数差异表达的EST与数据库中的序列没有明显的相似性，可能代表了与再生相关的新基因。然而，其他EST与已知参与再生相关过程、伤口愈合、细胞增殖、分化、形态可塑性、细胞存活、应激反应、免疫挑战和肿瘤转化的基因相似。在那些已被证实的基因中，细胞骨架基因，如肌动蛋白，以及发育基因，如Wnt和Hox基因，在再生过程中显示出有趣的表达谱。我们的发现为未来研究肠道再生的分子基础奠定了基础。此外，它还促进了棘皮动物在再生生物学中的应用，这些动物由于其惊人的特性和关键的进化地位，可能为研究再生过程的遗传基础提供重要线索。
Among deuterostomes, the regenerative potential is maximally expressed in echinoderms, animals that can quickly replace most injured organs. In particular, sea cucumbers are excellent models for studying organ regeneration since they regenerate their digestive tract after evisceration. However, echinoderms have been sidelined in modern regeneration studies partially because of the lack of genome-wide profiling approaches afforded by modern genomic tools. For the last decade, our laboratory has been using the sea cucumber Holothuria glaberrima to dissect the cellular and molecular events that allow for such amazing regenerative processes. We have already established an EST database obtained from cDNA libraries of normal and regenerating intestine at two different regeneration stages. This database now has over 7000 sequences. In the present work we used a custom-made microchip from Agilent with 60-mer probes for these ESTs, to determine the gene expression profile during intestinal regeneration. Here we compared the expression profile of animals at three different intestinal regeneration stages (3-, 7- and 14-days post evisceration) against the profile from normal (uneviscerated) intestines. The number of differentially expressed probes ranged from 70% at p < 0.05 to 39% at p < 0.001. Clustering analyses show specific profiles of expression for early (first week) and late (second week) regeneration stages. We used semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR) to validate the expression profile of fifteen microarray detected differentially expressed genes which resulted in over 86% concordance between both techniques. Most of the differentially expressed ESTs showed no clear similarity to sequences in the databases and might represent novel genes associated with regeneration. However, other ESTs were similar to genes known to be involved in regeneration-related processes, wound healing, cell proliferation, differentiation, morphological plasticity, cell survival, stress response, immune challenge, and neoplastic transformation. Among those that have been validated, cytoskeletal genes, such as actins, and developmental genes, such as Wnt and Hox genes, show interesting expression profiles during regeneration. Our findings set the base for future studies into the molecular basis of intestinal regeneration. Moreover, it advances the use of echinoderms in regenerative biology, animals that because of their amazing properties and their key evolutionary position, might provide important clues to the genetic basis of regenerative processes.
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影响因子：
0.9
作者：
Azumi, K;Takahashi, H;Satoh, N
通讯作者：
Satoh, N
影响因子：
2.4
作者：
Bannister, R;McGonnell, IM;Beesley, PW
通讯作者：
Beesley, PW
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
1.6
作者：
Chera, Simona;Kaloulis, Kostas;Galliot, Brigitte
通讯作者：
Galliot, Brigitte
影响因子：
2.5
作者：
Batts, Lorene E.;Polk, D. Brent;Kulessa, Holger
通讯作者：
Kulessa, Holger