Comparison of biomaterial delivery vehicles for improving acute retention of stem cells in the infarcted heart.
Comparison of biomaterial delivery vehicles for improving acute retention of stem cells in the infarcted heart.
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DOI：
10.1016/j.biomaterials.2014.04.114
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发表时间：
2014-08
期刊：
影响因子：
14
通讯作者：
Mooney, David J.
中科院分区：
文献类型：
--
作者：
Roche, Ellen T.;Hastings, Conn L.;Lewin, Sarah A.;Shvartsman, Dmitry E.;Brudno, Yevgeny;Vasilyev, Nikolay V.;O'Brien, Fergal J.;Walsh, Conor J.;Duffy, Garry P.;Mooney, David J.
Cell delivery to the infarcted heart has emerged as a promising therapy, but is limited by very low acute retention and engraftment of cells. The objective of the study was to compare a panel of biomaterials to evaluate if acute retention can be improved with a biomaterial carrier. Cells were quantified post-implantation in a rat myocardial infarct model in five groups (n=7–8); saline injection (current clinical standard), two injectable hydrogels (alginate, chitosan/β-glycerophosphate) and two epicardial patches (alginate, collagen). Human mesenchymal stem cells (hMSCs) were delivered to the infarct border zone with each biomaterial. At 24 hours, retained cells were quantified by fluorescence. All biomaterials had superior fluorescence to saline control, with 8 and 14-fold increases with alginate and chitosan/β-GP injectables, and 47 and 59-fold increases achieved with collagen and alginate patches, respectively. Immunohistochemical analysis qualitatively confirmed these findings. All four biomaterials retained 50–60% of cells that were present immediately following transplantation, compared to 10% for the saline control. In conclusion, all four injectable hydrogels and epicardial patches were demonstrated to more efficiently deliver and retain cells when compared to a saline control. Biomaterial-based delivery approaches show promise for future development of efficient in vivo delivery techniques.
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影响因子：
37.8
作者：
Hofmann, M;Wollert, KC;Drexler, H
通讯作者：
Drexler, H
DOI：
10.1073/pnas.192291499
发表时间：
2002-09-17
影响因子：
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影响因子：
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