The IGF1 small dog haplotype is derived from Middle Eastern grey wolves.
The IGF1 small dog haplotype is derived from Middle Eastern grey wolves.
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DOI：
10.1186/1741-7007-8-16
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发表时间：
2010-02-24
期刊：
影响因子：
5.4
通讯作者：
Wayne RK
中科院分区：
文献类型：
--
作者：
Gray MM;Sutter NB;Ostrander EA;Wayne RK
含有胰岛素样生长因子 1 (IGF1) 基因的选择性扫描与家犬的体型变化有关。 IGF1 的内含子 2 含有所有小型犬品种中都存在的 SINE 元件和单核苷酸多态性 (SNP)，而大型犬品种中几乎完全不存在。在这项研究中，我们调查了灰狼种群的大样本，以更好地了解 IGF1 变异的祖先模式，特别关注小型犬单倍型的分布及其与狗起源的关系。我们提供的 DNA 序列数据证实了灰狼大样本中 IGF1 内含子 2 区域不存在衍生的小 SNP 等位基因，并进一步证实所有野生犬科动物和大多数大型犬种中不存在小型犬相关的 SINE 元件。来自中东的灰狼单倍型具有较高的核苷酸多样性，表明其起源于那里。此外，PCA 和系统发育分析表明小型家犬 IGF1 单倍型与中东灰狼的 IGF1 单倍型有更密切的亲缘关系。灰狼中同时缺乏 SINE 元件和 SNP 等位基因，表明体型较小的突变晚于狗的驯化。然而，由于所有小型犬都具有这些诊断性突变，因此这些突变可能在家犬历史的早期就出现了。我们的结果表明，小型犬单倍型与中东狼的单倍型密切相关，并且与中东小型犬单倍型的古老起源一致。因此，根据过去的考古研究，我们的分子分析与中东狗小型体型的早期进化是一致的。请参阅 Driscoll 和 Macdonald 的相关意见：http://jbiol.com/content/9/2/10
A selective sweep containing the insulin-like growth factor 1 (IGF1) gene is associated with size variation in domestic dogs. Intron 2 of IGF1 contains a SINE element and single nucleotide polymorphism (SNP) found in all small dog breeds that is almost entirely absent from large breeds. In this study, we surveyed a large sample of grey wolf populations to better understand the ancestral pattern of variation at IGF1 with a particular focus on the distribution of the small dog haplotype and its relationship to the origin of the dog. We present DNA sequence data that confirms the absence of the derived small SNP allele in the intron 2 region of IGF1 in a large sample of grey wolves and further establishes the absence of a small dog associated SINE element in all wild canids and most large dog breeds. Grey wolf haplotypes from the Middle East have higher nucleotide diversity suggesting an origin there. Additionally, PCA and phylogenetic analyses suggests a closer kinship of the small domestic dog IGF1 haplotype with those from Middle Eastern grey wolves. The absence of both the SINE element and SNP allele in grey wolves suggests that the mutation for small body size post-dates the domestication of dogs. However, because all small dogs possess these diagnostic mutations, the mutations likely arose early in the history of domestic dogs. Our results show that the small dog haplotype is closely related to those in Middle Eastern wolves and is consistent with an ancient origin of the small dog haplotype there. Thus, in concordance with past archeological studies, our molecular analysis is consistent with the early evolution of small size in dogs from the Middle East. See associated opinion by Driscoll and Macdonald: http://jbiol.com/content/9/2/10
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DOI：
10.1126/science.1165448
发表时间：
2009-03-06
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Anderson TM;vonHoldt BM;Candille SI;Musiani M;Greco C;Stahler DR;Smith DW;Padhukasahasram B;Randi E;Leonard JA;Bustamante CD;Ostrander EA;Tang H;Wayne RK;Barsh GS
通讯作者：
Barsh GS
影响因子：
4.5
作者：
Cambray, Guillaume;Mazel, Didier
通讯作者：
Mazel, Didier
影响因子：
10.7
作者：
Pang JF;Kluetsch C;Zou XJ;Zhang AB;Luo LY;Angleby H;Ardalan A;Ekström C;Sköllermo A;Lundeberg J;Matsumura S;Leitner T;Zhang YP;Savolainen P
通讯作者：
Savolainen P
影响因子：
64.8
作者：
DAVIS, SJM;VALLA, FR
通讯作者：
VALLA, FR
影响因子：
14.9
作者：
Nickerson, DA;Tobe, VO;Taylor, SL
通讯作者：
Taylor, SL