Intrauterine infusion of low levels of interferon‐tau on day‐8 post‐estrus stimulates the bovine endometrium to secrete apolipoprotein‐A1: A possible implication for early embryo tolerance
Intrauterine infusion of low levels of interferon‐tau on day‐8 post‐estrus stimulates the bovine endometrium to secrete apolipoprotein‐A1: A possible implication for early embryo tolerance
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发情后第 8 天宫内输注低水平干扰素 tau 会刺激牛子宫内膜分泌载脂蛋白 A1：对早期胚胎耐受的可能影响
DOI：
10.1111/aji.13592
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发表时间：
2022
影响因子：
3.6
通讯作者：
Miyamoto Akio
中科院分区：
文献类型：
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作者：
Rashid Mohammad B.;Marey Mohamed A.;Fukuda Kenji;Haneda Shingo;Kusama Kazuya;Shimada Masayuki;Imakawa Kazuhiko;Miyamoto Akio
我们先前报道，来源于第7天胚泡的干扰素-tau(IFNT)在体外对牛子宫内膜上皮细胞(BEECs)产生抗炎反应。然而，早期胚胎来源的IFNT在体内对子宫蛋白质组图谱的真正影响大多是未知的。本研究旨在研究在发情后第8天注入低生理水平的无胚胎IFNT时子宫冲洗(UF)的蛋白质组学变化及其对子宫免疫微环境的可能影响。首先，在第6天向子宫腔内注入新鲜培养液，24小时后获得UF，并在第7天重复这一过程(对照UF)。第8天，在含有重组牛IFNT(100pg/ml)(添加IFNT的UF)的培养液中进行这一过程。检测对照组和添加IFNT的UF患者外周血单个核细胞(PBMC)的免疫反应。实时荧光定量聚合酶链式反应结果显示，添加IFNT的UF可下调PBMC中促炎细胞因子(TNFA、IL1B)的表达，上调抗炎细胞因子(TGFB1)和PTGE的表达。通过双向PAGE和TOF/TOF质谱仪检测，证实了添加IFNT的超滤液中有载脂蛋白-A1(Apo-A1)的存在，并通过酶联免疫吸附分析证实了这一点。蛋白质组学分析再次表明，IFNT体外刺激BEECs可上调Apo-A1的表达。此外，重组牛Apo-A1刺激PBMCs可下调PBMCs中TNFA1和NFKBand的表达，上调PBMCs中TGFB1和PTGE的表达。总之，我们的结果表明，在没有胚胎的情况下，微量的IFNT单独刺激子宫内膜上皮细胞分泌Apo-A1，从而启动抗炎环境，这可能为子宫接受早期胚胎铺平道路。
We previously reported that interferon‐tau (IFNT), derived from day‐7 blastocyst, generates anti‐inflammatory responses in bovine endometrial epithelial cells (BEECs) in vitro. However, the real in vivo impact of early embryo‐derived IFNT on the uterine proteomic profile is mostly unknown. This study aimed to investigate proteomic changes of uterine flush (UF) when infused with a low physiological level of IFNT without embryo on day‐8 post‐estrus and its possible impact on the uterine immunological microenvironment. First, a fresh medium was infused into the uterine lumen on day‐6, from which UF was obtained 24 h later, and this procedure was repeated on day‐7 (control UF). On day‐8, this procedure was done with a medium containing recombinant bovine IFNT (100 pg/ml) (IFNT‐supplemented UF). Control and IFNT‐supplemented UF were tested for immune responses in peripheral blood mononuclear cells (PBMCs). Real‐time PCR results revealed that IFNT‐supplemented UF downregulated pro‐inflammatory cytokines (TNFA, IL1B)and upregulated anti‐inflammatory cytokine (TGFB1)andPTGESin PBMCs. Through 2‐D PAGE, followed by TOF/TOF mass spectrometer, apolipoprotein‐A1 (Apo‐A1) protein was identified in the IFNT‐supplemented UF, which was confirmed by ELISA analysis. Proteomic analysis revealed again that the in vitro stimulation of BEECs by IFNT upregulated Apo‐A1 expression. Further, stimulation of PBMCs with recombinant bovine Apo‐A1 downregulatedTNFAandNFKBand upregulatedTGFB1andPTGESin PBMCs. Altogether, our results suggest that minute amounts of IFNT alone, normally secreted from bovine blastocyst, stimulate Apo‐A1 secretion from the endometrial epithelium in the absence of embryo that initiates an anti‐inflammatory environment, which could pave the way for the acceptance of early embryo in the uterus.
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影响因子：
3.6
作者：
K. Nagaoka;A. Sakai;H. Nojima;Y. Suda;Y. Yokomizo;K. Imakawa;S. Sakai;R. Christenson
通讯作者：
K. Nagaoka;A. Sakai;H. Nojima;Y. Suda;Y. Yokomizo;K. Imakawa;S. Sakai;R. Christenson
影响因子：
3.7
作者：
Kusama K;Rashid MB;Kowsar R;Marey MA;Talukder AK;Nagaoka K;Shimada M;Khatib H;Imakawa K;Miyamoto A
通讯作者：
Miyamoto A
影响因子：
2.8
作者：
Thatcher, WW;Guzeloglu, A;Pru, JK
通讯作者：
Pru, JK
妊娠早期绵羊胸腺ISG15、孕激素受体及孕激素诱导阻断因子表达的变化
影响因子：
2.8
作者：
Leying Zhang;Jie Xue;Qingkai Wang;Wan Lv;Hao Mi;Yong Liu;Ling Yang
通讯作者：
Ling Yang
DOI：
10.1016/j.anireprosci.2004.04.014
发表时间：
2004-07-05
期刊：
Reproductive biology and endocrinology : RB&E
影响因子：
--
作者：
Spencer, Thomas E;Bazer, Fuller W
通讯作者：
Bazer, Fuller W