Day 7 Embryos Change the Proteomics and Exosomal Micro-RNAs Content of Bovine Uterine Fluid: Involvement of Innate Immune Functions.
Day 7 Embryos Change the Proteomics and Exosomal Micro-RNAs Content of Bovine Uterine Fluid: Involvement of Innate Immune Functions.
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DOI：
10.3389/fgene.2021.676791
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发表时间：
2021
影响因子：
3.7
通讯作者：
Miyamoto A
中科院分区：
文献类型：
--
作者：
Kusama K;Rashid MB;Kowsar R;Marey MA;Talukder AK;Nagaoka K;Shimada M;Khatib H;Imakawa K;Miyamoto A
本研究旨在使用胚胎移植计划的胚胎供体奶牛来表征与第 7 天 (D7) 妊娠相关的奶牛子宫冲洗 (UF) 中的蛋白质和外泌体 microRNA (miRNA)。超数排卵的奶牛要么进行授精（AI 奶牛），要么保持不授精（Ctrl 奶牛）。在存在多个胚胎（AI 牛）或没有胚胎（Ctrl 牛）的情况下在 D7 收集 UF，并进行同量异位标签以进行相对和绝对定量蛋白质分析。总共鉴定出 336 种蛋白质，其中 260 种蛋白质在 AI 奶牛中比 Ctrl 奶牛高出 2 倍多。基因本体分析显示，许多差异表达蛋白涉及“中性粒细胞相关”和“细胞外囊泡外泌体相关”术语。对 AI UF 中较高浓度的蛋白质进行计算机分析，鉴定出 18 种独特表达的蛋白质。从 UF 中分离出外泌体，对其中的 RNA 进行 miRNA-seq，鉴定出 37 个 miRNA。其中，AI 牛的 UF 中 3 个 miRNA 较低，6 个 miRNA 高于 Ctrl 牛。主成分分析显示 bta-miR-29a、bta-miR-199b、SUGT1 和 PPID 之间的 miRNA 和蛋白质密切相关。此外，受试者工作特征曲线分析表明 SUGT1 是子宫中胚胎存在的最佳预测因子。这些发现表明，子宫中存在多个 D7 胚胎可导致 UF 的蛋白质组成和外泌体 miRNA 含量发生显着变化，从而介导牛孵化前胚胎与子宫之间的先天免疫相互作用。
This study aimed to characterize proteins and exosomal microRNAs (miRNAs) in the uterine flushings (UF) of cows associated with Day 7 (D7) pregnancy using the embryo donor cows of the embryo transfer program. Superovulated cows either were inseminated (AI cows) or remained non-inseminated (Ctrl cows). UF was collected on D7 in the presence of multiple embryos (AI cows) or without embryos (Ctrl cows) and subjected to isobaric tags for relative and absolute quantification protein analysis. A total of 336 proteins were identified, of which 260 proteins were more than 2-fold higher in AI cows than Ctrl cows. Gene ontology analysis revealed that many differentially expressed proteins were involved in “neutrophil-related” and “extracellular vesicular exosome-related” terms. In silico analysis of proteins with higher concentrations in the UF of AI identified 18 uniquely expressed proteins. Exosomes were isolated from the UF, from which RNA was subjected to miRNA-seq, identifying 37 miRNAs. Of these, three miRNAs were lower, and six miRNAs were higher in the UF of AI cows than those of Ctrl ones. The principal component analysis displayed a close association in miRNA and protein between bta-miR-29a, bta-miR-199b, SUGT1, and PPID. In addition, the receiver operating characteristic curve analysis showed that SUGT1 was the best predictor for the presence of embryos in the uterus. These findings suggest that the presence of multiple D7 embryos in the uterus can lead to significant changes in the protein composition and exosomal miRNA contents of UF, which could mediate innate immunological interactions between the pre-hatching embryo and the uterus in cows.
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影响因子：
3.7
作者：
Forde N;Simintiras CA;Sturmey R;Mamo S;Kelly AK;Spencer TE;Bazer FW;Lonergan P
通讯作者：
Lonergan P
影响因子：
4.8
作者：
Muralimanoharan, Sribalasubashini;Kwak, Youn-Tae;Mendelson, Carole R.
通讯作者：
Mendelson, Carole R.
影响因子：
4.2
作者：
Gross N;Kropp J;Khatib H
通讯作者：
Khatib H
影响因子：
3.6
作者：
Forde, N.;Carter, F.;Roche, J. F.
通讯作者：
Roche, J. F.
影响因子：
3.7
作者：
Gross N;Kropp J;Khatib H
通讯作者：
Khatib H