Whole blood immunophenotyping uncovers immature neutrophil-to-VD2 T-cell ratio as an early marker for severe COVID-19.
Whole blood immunophenotyping uncovers immature neutrophil-to-VD2 T-cell ratio as an early marker for severe COVID-19.
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DOI：
10.1038/s41467-020-19080-6
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发表时间：
2020-10-16
影响因子：
16.6
通讯作者：
Ng LF
中科院分区：
文献类型：
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作者：
Carissimo G;Xu W;Kwok I;Abdad MY;Chan YH;Fong SW;Puan KJ;Lee CY;Yeo NK;Amrun SN;Chee RS;How W;Chan S;Fan BE;Andiappan AK;Lee B;Rötzschke O;Young BE;Leo YS;Lye DC;Renia L;Ng LG;Larbi A;Ng LF
SARS-CoV-2是导致当前新冠肺炎大流行的新型冠状病毒。严重的并发症只在一小部分感染患者中观察到，但导致这种进展的细胞机制仍不清楚。对54名新冠肺炎患者的全血样本进行综合流式细胞术分析，发现未成熟的中性粒细胞数量急剧增加。这种增加与疾病的严重程度密切相关，并与IL-6和IP-10水平的升高有关，这是细胞因子风暴中的两个关键因素。CD8T细胞和VD2CD2γδT细胞的细胞数下降最为明显，两者都表现出分化和活化的增加。ROC分析显示，未成熟中性粒细胞与VD2(或CD8T细胞)的计数比预测肺炎的发生(0.9071)和缺氧的发生(0.8908)具有很高的敏感性和特异性。因此，它将是预防性患者管理和改善医疗资源管理的有用的预后标志。新冠肺炎的严重程度与细胞因子水平和淋巴细胞减少有关，但免疫细胞亚群的作用尚不清楚。在这里，作者对54例新冠肺炎患者的全血样本进行了免疫表型分析，发现未成熟的中性粒细胞/VD2T细胞比率与严重的新冠肺炎有关。
SARS-CoV-2 is the novel coronavirus responsible for the current COVID-19 pandemic. Severe complications are observed only in a small proportion of infected patients but the cellular mechanisms underlying this progression are still unknown. Comprehensive flow cytometry of whole blood samples from 54 COVID-19 patients reveals a dramatic increase in the number of immature neutrophils. This increase strongly correlates with disease severity and is associated with elevated IL-6 and IP-10 levels, two key players in the cytokine storm. The most pronounced decrease in cell counts is observed for CD8 T-cells and VD2 γδ T-cells, which both exhibit increased differentiation and activation. ROC analysis reveals that the count ratio of immature neutrophils to VD2 (or CD8) T-cells predicts pneumonia onset (0.9071) as well as hypoxia onset (0.8908) with high sensitivity and specificity. It would thus be a useful prognostic marker for preventive patient management and improved healthcare resource management. COVID-19 severity is associated with cytokine levels and lymphopenia, but the role of immune cell subsets is not well understood. Here the authors immunophenotype whole blood samples from 54 COVID-19 patients and find that the immature neutrophil-to-VD2 T-cell ratio is associated with severe COVID-19.
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